Expression Sample Clauses
The 'Expression' clause defines how parties may communicate their intentions, statements, or representations within the context of the agreement. Typically, this clause clarifies whether communications must be made in writing, can be delivered electronically, or may be expressed verbally, and may specify the required form or medium for such expressions to be considered valid. Its core practical function is to ensure that all parties understand the acceptable methods for expressing contractual intentions, thereby reducing ambiguity and potential disputes over the validity of communications.
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Expression. Each of the hybrid transcription units described above will be cloned in the shuttle vector yepl3. Each of the four plasmids will then be transformed into an appropriate leu2 recipient yeast strain. Yeast cultures grown in 4 percent glucose will be assayed for their content of insulin, proinsulin, or related polypeptides using column chromatography, RIA, and gel electrophoresis (western blot) assays. The objective of the research in phase 1 will be to achieve at least a minimal level (0.01 to 0.1 percent of total soluble protein) as intact intracellular insulin/proinsulin, or as a hybridprotein from which intact insulin/proinsulin can easily be obtained by enzymatic or chemical conversion. [*] designates portions of this document that have been omitted pursuant to a request for confidential treatment filed separately with the Commission. The objective of phase II is to construct a genetically stable yeast strain that accumulates intact human insulin/proinsulin or a hybrid protein from which intact insulin/proinsulin can easily be obtained by enzymatic or chemical conversion at [ * ] molecules per 1 at [ * ] cellprotein per 1. By combination of the following approaches this goal will be reached. - Variation in the plasmid vector, particularly as regards its copy number, replication control, segregation mechanism. - Chromosomal integration of the hybrid transcription unit at multiple sites. - Regulatory modulation of the expression of tpi/or other yeast promoter -- both by mutation and by cloning and over-production of gene products required for transcription. - Exploration of the effect of unfavorable codon usage in the proinsulin coding sequence upon expression of proinsulin in yeast. The remedy for this could be to construct a wholly or partially synthetic proinsulin gene with codons which are optimally used in yeast. - Explore the possibilities of using as host organism, other eukaryotic microorganism, that is acceptable for large scale production for NOVO. - Application of general principles for optimizing gene expression in yeast, e.g.: chloramphenical acetyl transferase -- attached in the context of many different sequences at crucial points in the translation unit. Phase III --------- The specific approaches to be followed in optimizing insulin secretion from yeast will depend strongly upon the relative efficacy of various leader peptide constructions used in the phase 1 experiments. In order to increase the level of insulin secretion from yeast, gen...
Expression. An open-array constructor consists of one or more expressions separated by commas and enclosed in square brackets. Each expression must be assignment compatible with the element type of the open-array parameter. The use of an open- array constructor corresponds to creating a temporary array variable, and initializing the elements of the temporary array with the values given by the list of expressions. For example, given the declaration of the Sum function above, the statement X := Sum([A, 3.14159, B + C]); corresponds to Temp[0] := A; Temp[1] := 3.14159; Temp[2] := B + C; X := Sum(Temp); where Temp is a temporary array variable with three elements of type Double. A type variant open-array parameter allows an open array of expressions of varying types to be passed to a procedure or function. A type variant open-array parameter is declared using the syntax The array of const syntax is analogous to array of TVarRec. The TVarRec type is a variant record type which can represent values of integer, boolean, character, real, string, pointer, class, and class reference types. The TVarRec type is declared in the System unit as follows TVarRec = record case VType: Byte of vtInteger: (VInteger: Longint); vtBoolean: (VBoolean: Boolean); vtChar: (VChar: Char); vtExtended: (VExtended: PExtended); vtString: (VString: PString); vtPointer: (VPointer: Pointer); vtPChar: (VPChar: PChar); vtObject: (VObject: TObject); vtClass: (VClass: TClass); The VType field determines which value field is currently defined. Notice that for real or string values, the VExtended or VString field contains a pointer to the value rather than the value itself. The vtXXXX value type constants are also declared in the System unit: vtInteger = 0; vtBoolean = 1; vtChar = 2; vtExtended = 3; vtString = 4; vtPointer = 5; vtPChar = 6; vtObject = 7; vtClass = 8; The MakeStr function below takes a type variant open-array parameter and returns a string that is the concatenation of the string representations of the arguments. The AppendStr, IntToStr, FloatToStr, and StrPas functions used by MakeStr are defined in the SysUtils unit. function MakeStr(const Args: array of const): string; const BoolChars: array[Boolean] of Char = ('F', 'T');
Expression. It includes preliminary and final products and any materials and information developed for the purposes of producing those final products. "Works" does not include articles submitted to peer review or reference journals or independent research projects.
Expression. Employee demonstrates the ability to convey ideas and suggestions orally and in writing in a manner appropriate to the situation and his/her position.
Expression. Add the date when the rental period begins. This section states that it will be a monthly contract.
Expression. The expression must be assignment-compatible with the type of the variable or the type of the function result. See the section “Type compatibility” on page 25. Some examples of assignment statements follow:
Expression. The pair/group must demonstrate the ability to project the emotion of the music to the audience throughout. Movements must flow and be performed with amplitude, and synchronicity using the maximum amount of space and floor possible. Choreography must demonstrate imagination, originality in composition and in entries /exits.
Expression. The expression of a luciferase reporter containing 3’UTR of Kv4.2 could be influenced by overexpression or inhibition of miR-324-5p (Fig. 3-1). Furthermore, miR-324-5p regulates endogenous Kv4.2 protein expression (Fig. 3-4A and
Expression use of non-verbal behaviour to solicit student attention and interest
10. Speaks in a dramatic expressive way.
11. Moves about while lecturing.
12. Gestures with hands or arms.
13. Makes eye contact with students.
14. Gestures with head or body.
15. Tells jokes or humorous anecdotes.
16. Effectively uses prepared notes or text.
17. Smiles or laughs while teaching.
18. Avoids distracting mannerisms.
Expression. During differentiation the expression of Stro-1 decreases, whilst the expression of osteoblast markers such as ALP increases (▇▇▇▇▇, ▇▇▇▇▇▇▇▇▇ et al. 2000, ▇▇▇▇▇ and ▇▇▇▇▇▇▇▇ 2006). Pre-osteoblasts are committed to osteoblastic differentiation, but still retain some proliferative capacity. Osteoblasts are the cells responsible for deposition and maintenance of skeletal tissues which function via introduction of extracellular proteins such as osteocalcin, and type I collagen. Osteoblasts may undergo apoptosis or become inactive and form bone-lining cells or embed in bone matrix and form osteocytes which are the most differentiated bone cells (▇▇▇▇▇▇, ▇▇▇▇▇▇ et al. 2006, Long 2011). Osteocytes are stellate cells with characteristic cellular processes which link with each other through canaliculi within the bone. Osteocytes constitute 95% of all bone cells and are embedded in mineralised matrix. Overall these cells, together are responsible for the regulation of bone remodelling and bone flexibility (▇▇▇▇▇▇, ▇▇▇▇▇▇ et al. 2006, ▇▇▇▇, ▇▇▇▇ et al. 2009). Condensation Proliferation Early Differentiation Differentiation Commitment Differentiation BMP2 Stro1 BMP2 BMP2 PTH Mesenchymal Osteoprogenitor Pre-osteoblast Osteoblast Stem Cell Osteocyte RUNX2 Osterix RUNX2 Osterix ALP Coll 1 OPN RUNX2 Osterix ALP Coll 1 OPN BSP OSC OSC Figure 1-1; Osteoblastogenesis starting from Mesenchymal Stem Cells to Osteocyte formation Regulation of osteoblast differentiation is under the control of a variety of factors including hormones, transcription factors, growth factors, cytokines and miRNAs. These factors on the other hand, can modulate each other’s function and make a network to tune osteogenesis (Figure1-1). • Hormones The involvement of hormones in osteoblast differentiation and bone mass density has been well known for a long time. For instance, recombinant homologous growth hormone was shown to accelerate ossification when administrated systemically (▇▇▇▇▇▇▇, Bail et al. 1999). Parathyroid hormone was demonstrated as an enhancer of total-body bone mineral density dose dependently following daily subcutaneous injection in postmenopausal women (▇▇▇▇, ▇▇▇▇▇▇ et al. 2001). Estrogen, which is also known as 17β-estradiol or E2, was demonstrated to promote osteogenesis by up regulation of ALP activity, RUNX2 and parathyroid hormone, whilst inhibiting adipogenesis by reducing PPARƔ, AP2 and lipoprotein lipase (LPL) expression in KS483 cell line (Dang, ▇▇▇ ▇▇▇▇▇▇▇▇▇ et al. 2002)....