Expression Sample Clauses

Expression. Meaning Expression Meaning
Expression. This Agreement, together with the Indenture, the Security Agreements and any other agreement executed in connection herewith or therewith, is intended by the parties as a final expression of this Agreement and is intended as a complete and exclusive statement of the terms and conditions hereof.
Expression. An expression, sometimes designated version or variant, is (one of) the realization(s) of a work in a specific collection of actual words, sentences, paragraphs, etc. that result in the form of text made by the originator of the expression. In some cases it will be necessary to deal with structured text like tables and lists, but the physical form aspects, as typeface or page-layout, must be excluded from the expression level whenever possible. For instance, each consolidation of a formal act of law is an expression of that work. For instance, the English, Dutch, Italian, and German versions of a European directive are different expressions of the same work. The concept of expression was taken from the IFLA-FRBR.
Expression. The terms and expressions referred to in this agreement shall have the same meaning with those used in the Loan Agreement.
Expression. We measured expression of CHRNA5 in two tissues to address whether genetic variants in the cluster are associated with expression regulation. In particular, rs3841324 has been reported as a promoter regulatory element in cell culture(99). We sought to test the effect of this variant on expression in vivo. Expression of CHRNA5 was strongly associated with rs3841324 genotype, with relative expression levels higher for the short allele in blood (r=0.72, p=4x10-71) and subcutaneous adipose tissue (r=0.73, p=2x10-63; See Figure 3.2). Association with expression of CHRNA5 was also examined for the other SNPs within the LD block, with one marker from each equivalence class tested (see Table 3.5). All markers were significantly associated with expression. Adjusting for rs3841324 reduces the significance of the association for the other SNPs drastically, in subcutaneous adipose tissue only rs1051730 remains nominally significant (p=0.018) and three SNPs show nominally significant association in blood (minimum p=0.006 for rs1051730). Overall, expression in blood and subcutaneous adipose tissue is strongly associated with rs3841324. However, we cannot rule out an additional comparably weak effect of another SNP, which was best captured by rs1051730. Expression of CHRNA5 was not associated with lifetime regular smoking, or with smoking within the past 24 hours (data not shown). We have established that there is no risk for nicotine dependence or lung cancer associated with this variant independent of the risk associated with rs1051730 (See Table 3.4). However, there is strong LD between the two variants. The T allele of rs1051730 only appears on the haplotype background including the long, or low expression, allele of rs3841324. A careful characterization of the CHRNA5/CHRNA3/CHRNB4 cluster does not identify any variants with stronger association to nicotine dependence or lung cancer than rs1051730/rs16969968. Therefore the SNP non-synonymous SNP rs16969968 remains the variant most likely to have functional effects leading to the observed association signals within this region.
Expression. Each of the hybrid transcription units described above will be cloned in the shuttle vector yepl3. Each of the four plasmids will then be transformed into an appropriate leu2 recipient yeast strain. Yeast cultures grown in 4 percent glucose will be assayed for their content of insulin, proinsulin, or related polypeptides using column chromatography, RIA, and gel electrophoresis (western blot) assays. The objective of the research in phase 1 will be to achieve at least a minimal level (0.01 to 0.1 percent of total soluble protein) as intact intracellular insulin/proinsulin, or as a hybridprotein from which intact insulin/proinsulin can easily be obtained by enzymatic or chemical conversion. [*] designates portions of this document that have been omitted pursuant to a request for confidential treatment filed separately with the Commission. Phase II -------- The objective of phase II is to construct a genetically stable yeast strain that accumulates intact human insulin/proinsulin or a hybrid protein from which intact insulin/proinsulin can easily be obtained by enzymatic or chemical conversion at [ * ] molecules per 1 at [ * ] cellprotein per 1. By combination of the following approaches this goal will be reached. - Variation in the plasmid vector, particularly as regards its copy number, replication control, segregation mechanism. - Chromosomal integration of the hybrid transcription unit at multiple sites. - Regulatory modulation of the expression of tpi/or other yeast promoter -- both by mutation and by cloning and over-production of gene products required for transcription. - Exploration of the effect of unfavorable codon usage in the proinsulin coding sequence upon expression of proinsulin in yeast. The remedy for this could be to construct a wholly or partially synthetic proinsulin gene with codons which are optimally used in yeast. - Explore the possibilities of using as host organism, other eukaryotic microorganism, that is acceptable for large scale production for NOVO. - Application of general principles for optimizing gene expression in yeast, e.g.: chloramphenical acetyl transferase -- attached in the context of many different sequences at crucial points in the translation unit. Phase III --------- The specific approaches to be followed in optimizing insulin secretion from yeast will depend strongly upon the relative efficacy of various leader peptide constructions used in the phase 1 experiments. In order to increase the level of insulin secreti...
Expression. Expression improvements can be explored by Eleven’s Espresso suite of expression vectors and codon optimization. If the glycosylation state of a lead needs to be changed (i.e. to decrease or eliminate glycosylation), point mutants can be created and evaluated.
Expression. You have the right to hold opinions and express your views individually or with others. Everyone has the right to assemble with other people in a peaceful way.