DNA extraction Sample Clauses

DNA extraction. Well water- DNA was extracted according to reference1. Filters were aseptically removed from casing and cut into pieces, and processed using the UltraCleanTM Water DNA Isolation kit (Mo Bio) according to the manufacturer’s instructions using the alternative lysis method (65 °C incubation for 10 minutes). DNA was stored at −20 °C until analysis. Drill-wetting mud- DNA was extracted using the Omega X.X.X.X.XX Soil DNA Kit. Samples were processed according to manufacturer’s instructions using 300 mg of starting material, vortexing for 5 minutes to lyse samples, and eluting in a final volume of 50 L of elution buffer supplied with the kit. DNA was stored at −20 °C until analysis.
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DNA extraction. The Nucleon II kit was used according to the manufacturer’s instructions (see appendix).
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DNA extraction. The majority of samples that pass pathology review are able to have DNA isolated. A small percentage of samples yield <50 ng of DNA, which is currently the minimum amount of DNA needed for the FMI assay. If this occurs, the Sponsor Contact is contacted to decide if additional sample is available, and, in consultation with FMI decide whether to attempt to continue the assay.
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DNA extraction. For the new samples for which no DNA was available, DNA was extracted from newly collected samples. DNA was extracted using the Wizard® Genomic DNA Purification kit (Promega, WI, USA) following manufacturer´s instructions for “Isolating Genomic DNA from Tissue Culture Cells and Animal Tissue”. The starting material was approximately 20 mg of tissue and after extraction all samples were suspended in equal volumes of Milli-Q water. DNA quantity (ng/µl) was evaluated on the Qubit® 2.0 Fluorometer (Life Technologies) and DNA integrity was assessed by electrophoresis.
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DNA extraction. Completed Sample Prep •Add detergent •Incubate at 60 C •Add potassium acetate •Incubate at 4C •Centrifuge •Remove supernatant •Add ice cold isopropanol to precipitate DNA •Centrifuge •Wash with 70% ethanol •Dry DNA pellet •Dissolve DNA in water Real Time PCR Can’t test every tree in the grove Please be reasonable with samples Number of Samples Processed • To date: 40,545 grower samples + ~15,000 internal samples A Xxxxx a Day and then Doubled.... Would you rather have a check for $100K or $0.01 to start and then doubled each day for 30 days? Real Time PCR Lab Report CT< 30 Positive CT between 30-32 Questionable CT>32 No HLB Found Threshold Determination Reasons to Send in Samples • Determine if you have HLB – get on a control/management program • Train your scouts • Quality control of the scouting crews • Research Differences Between Crews and Areas Questions • Contact Information: Xxxx Xxxx United States Sugar Corporation Technical Operations 000 Xxxxx xx Xxxx Ave. Clewiston, FL 33440 000-000-0000 000-000-0000 (cell) xxxxxx@xxxxxxx.xxx • New Shipping Address: Technical Operations Laboratory United States Sugar Corporation 0000 Xxxxx X.X. Owen Ave Clewiston, FL, 33440 xxxx://xxx.xxxxxxxxxxxxxx.xxx/files/712432d4-2860-44d1-a.pdf xxxx://xxx.xxxxx.xxx/posgreen/061221mikeirey.pdf
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DNA extraction. DNA was extracted from the xxxxx xxxxx using the Illustra blood genomicprep spin kit, (GE Healthcare, UK).
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DNA extraction. DNA extraction from xxxxx xxxxx of 466 RISCK subjects and 310 MARINA subjects was carried out using an Illustra blood genomic prep mini spin kit (GE healthcare, UK). Xxxxx xxxxx in EDTA had been frozen since preparation of blood samples. Genomic DNA was purified from 200 μl buffy coat. This process was carried out according to manufacturer’s instructions. This includes the below steps:
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Related to DNA extraction

  • Production Lessee shall, subject to applicable laws, regulations and orders, operate and produce all xxxxx upon the leased land so long as the same are capable of producing in paying quantities, and shall operate the same so as to produce at a rate commensurate with the rate of production of xxxxx on adjoining lands within the same field and within the limits of good engineering practice, except for such times as there exist neither market nor storage therefore, and except for such limitations on, or suspensions of, production as may be approved in writing by Lessor. Lessee shall be responsible for adequate site security on all producing properties.

  • Development Records Each Party shall maintain complete, current and accurate records of all Development activities conducted by it hereunder, and all data and other information resulting from such activities. Such records shall fully and properly reflect all work done and results achieved in the performance of the Development activities in good scientific manner [*]. Each Party shall document all non-clinical studies and clinical trials in formal written study reports according to applicable Laws and national and international guidelines (e.g., ICH, GCP, GLP, and GMP). Each Party shall have the right to review and copy such records maintained by the other Party at reasonable times and to obtain access to the original [*].

  • Project Completion Part 1 – Material Completion

  • Plant The expression ‘Plant’ as used in the tender papers shall mean every temporary accessory necessary or considered necessary by the Engineer to execute, construct, complete and maintain the work and all altered, modified, substituted and additional works ordered in the time and the manner herein provided and all temporary materials and special and other articles and appliance of every sort kind and description whatsoever intended or used therefore.

  • Validation To validate the notice requirements outlined in Section 5.3, the Assuming Institution shall provide the Receiver (i) an Affidavit of Publication to meet the publication requirements outlined in Section 5.3(a) and (ii) the Assuming Institution will prepare an Affidavit of Mailing in a form substantially similar to Exhibit 2.3B after mailing the seven (7) day Notice to Depositors as required under Section 5.3(b).

  • Samples Physical examples of materials, equipment, or workmanship that are representative of some portion of the Work and that establish the standards by which such portion of the Work will be judged. The Contractor shall furnish for approval all samples required by the Contract Documents. The Work shall be in accordance with approved samples.

  • Production Work The Company may use Outside Entities to perform production work outside the plant and its environs provided the Company demonstrates that it is utilizing plant equipment to the maximum extent consistent with equipment capability and customer requirements and the Company is making necessary capital investments to remain competitive in the steel business and is in compliance with Article Eleven, Section B (Investment Commitment).

  • Project 3.01. The Recipient declares its commitment to the objectives of the Project. To this end, the Recipient shall carry out the Project in accordance with the provisions of Article IV of the General Conditions.

  • Commissioning Commissioning tests of the Interconnection Customer's installed equipment shall be performed pursuant to applicable codes and standards. If the Interconnection Customer is not proceeding under Section 2.3.2, the Utility must be given at least ten (10) Business Days written notice, or as otherwise mutually agreed to by the Parties, of the tests and may be present to witness the commissioning tests.

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