Material and Methods. All requests to perform Work in, upon and along road right-of-way must be approved by the County Engineer.
Material and Methods. A. Participants We included 72 randomized control trial Kenyan infants that were studied in [3][4]. All subjects participated in the study should have the following scale: • Age, between 6.5 to 9.5 months. • Never had a chronic or acute disease. • Never consumed vitamin and mineral in the past 8 weeks. • Never had antibiotics in the past 10 weeks.
Material and Methods. 2.1 Chemicals and materials LC-MS-grade acetonitrile (ACN) and formic acid were purchased from Biosolve B.V. (Valkenswaard, Netherlands). Anhydrous methyl tert-butyl ether (MTBE, ≥99.8%), ammonium acetate (≥99.0%) and ammonium formate (≥99.9%) were purchased from Sigma-Xxxxxxx (St. Xxxxx, Missouri, United States). Purified water was obtained from a Milli-Q PF Plus system (Merck Millipore, Burlington, Massachusetts, United States). The standard reagents α-linolenoyl ethanolamide (α-LEA), palmitoleoyl ethanolamide (POEA), pentadecanoyl ethanolamide (PDEA), linoleoyl ethanolamide (LEA), anandamide (AEA), docosahexaenoyl ethanolamide (DHEA), 1-arachidonoylglycerol (1-AG), 2- arachidonoylglycerol (2-AG), 1-linoleoyl glycerol (1-LG), 2-linoleoyl glycerol (2-LG), palmitoyl ethanolamide (PEA), dihomo-γ-linolenoyl ethanolamide (DGLEA), docosatetraenoyl ethanolamide (DEA), 1-oleoyl glycerol (1-OG), 2-oleoyl glycerol (2-OG), stearoyl ethanolamide (SEA), eicosapentaenoyl ethanolamide (EPEA), xxxx acid ethanolamide (ETAEA), N-oleoylethanolamine (OEA) and deuterated standards N-(2- hydroxyethyl-1,1,2,2-d4)-9Z,12Z-octadecadienamide (LEA-d4), N-(2-hydroxyethyl- 1,1',2,2'-d4)-4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenamide (DHEA-d4), N-(2- hydroxyethyl)-5Z,8Z,11Z,14Z-eicosatetraenamide-5,6,8,9,11,12,14,15-d8 (AEA-d8), 5Z,8Z,11Z,14Z-eicosatetraenoic-5,6,8,9,11,12,14,15-d8 acid (2-AG-d8), N-(2- hydroxyethyl)-hexadecanamide-7,7,8,8-d4 (PEA-d4), N-(2-hydroxyethyl)-octadecanamide- 18,18,18-d3 (SEA-d3) and N-(2-hydroxyethyl-1',1,2,2'-d4)-9Z-octadecenamide (OEA-d4) were purchased from Cayman Chemical (Ann Arbor, Michigan, United States).
Material and Methods. Objective 1: Hybrid adaptation and evaluation Experiments will be conducted at Amarillo (irrigated), College Station (rainfed and irrigated), east Texas (China) (rainfed), and the Rio Grande Valley (Monte Alto) (irrigated). In each experiment, most advanced materials from the TAMU-Ceres biomass and sweet sorghum program will be planted in small plots (min. of 4 rows × 15’). Plants will be grown in 30-inch rows (Amarillo, College Station and China), or 40-inch rows (Monte Alto), respectively. Initially seeding rates for the different regions will be based on conventional forage sorghum guidelines. The fertilization requirement will be estimated based on the difference between nutrient measured in deep soil samples (N: 0’—3’; P, K, Micronutrients: 0-8”) and the plant requirement estimated by yield goal. Weed control will be atrazine applied preplant or early post emergence. Experiments requiring irrigation at Amarillo, College Station, and Monte Alto will be furrow irrigated. Insect control measures will be taken as warranted by pest pressure. At College Station, China, and Monte Alto all sorghum plants will be harvested for the first time when the head-producing hybrids are in the soft-dough growth stage. Thereafter a ratoon crop will be grown and all sorghum plants will be harvested when the head-producing plants reach the soft-dough growth stage for the second time (dependent on growing conditions and hybrid maturity, a third crop will be harvested at Monte Alto). At Amarillo, due to is shorter growing season, it is likely that only one harvest will be performed in late September. Agronomic data (such as plant height, lodging, biomass production, and plant moisture) and compositional data (analyzed at the TAMU sorghum quality lab for the biomass hybrids and sucrose yield and concentration for the sweet sorghums and nutrient removal of both the sweet and biomass sorghums) will be collected at all locations. Experimental design will be a randomized complete block with 4 replicates. Data will be subjected to ANOVA. Objective 2: Plant populations Experiments will be conducted at Amarillo (irrigated), College Station (rainfed and irrigated), China (rainfed), and Monte Alto (irrigated). With the exception of plant populations, agronomic practices will be as described for objective 1. In this study we will evaluate the effect of varying plant populations on the two most promising biomass sorghums and the two most promising sweet sorghums developed by the TAMU-Ce...
Material and Methods. All requests to perform work in, upon or along Highway right-of-ways must be approved by the District Engineer (and City). In Cities, Petitioner will obtain additional Permits, as required by City.
Material and Methods. Patient population Author Manuscript
Material and Methods. All elbow CT images acquired from dogs presenting to XXX between XXX and XXX were retrospectively retrieved from the image archive. Elbow images included both normal and abnormal elbows (affected by MCPD as confirmed by clinical findings, CT and arthroscopy). Image acquisition
Material and Methods. Participants A nationwide postal survey among all known patients with hemophilia, the Hemophilia in the Netherlands-5 study4, was conducted in the Netherlands in 2001, following four previous surveys dating back to 1972. Details on this survey have been described previously4. Patients included were listed with the Netherlands Hemophilia Society, with the hemophilia treatment centers, or were known from updated mailing lists from previous surveys. The response to the Hemophilia in the Netherlands-5 study was 70% (1066 out of 1519). For the present study we included all 733 participants who were between 15 and 64 years of age.
Material and Methods. Chemicals The perdeuterated FFA (DFFA) with chain length of C16:0 and C22:0 were obtained from Larodan (Malmö, Sweden). The DFFA with chain length of C18:0 and C20:0 were purchased from Cambridge Isotope laboratories (Andover, Massachusetts), while DFFA with chain length of C24:0 was obtained from ARC laboratories (Apeldoorn, The Netherlands). Cholesterol, protonated fatty acids and deuterated and protonated acetate buffer salts were purchased from Sigma-Xxxxxxx Chemie GmbH (Schnelldorf, Germany). Protonated and perdeuterated lipophilic moisturizers were kindly provided by Uniqema (Gouda, The Netherlands). Isolation and separation of porcine ceramides Fresh pig skin was obtained from a xxxxxxxxx house. Upon arrival the SC was isolated as described elsewhere (Xxxxxx et al., 1997). SC lipids were extracted using the method of Xxxxx and Xxxx (Xxxxx and Xxxx, 1959). The extracted lipids were applied on a silicagel 60 column and the various lipid classes were eluted sequentially using various solvent mixtures as published previously (Bouwstra et al., 1996a). The lipid composition of the collected fractions was established by one dimensional high performance thin layer chromatography, as described earlier (Xxxxxxxx et al., 1996a). For quantification, authentic standards were run in parallel. The quantification was performed after charring, using a photodensitometer with peak integration (Biorad, GS 710). The fractions containing ceramides (CER) were pooled and the resulting CER mix had a CER EOS (Xxxxx et al., 1993) content of approx. 12%, which is similar to the acylceramide content of human SC (Ponec et al., 2003a; Xxxxx et al., 1985). The remainder of the mix primarily consisted of CER NS (64%) with CER NP, AS (long chain FFA), AS(short chain FFA) and AP in concentrations of 8, 6, 4 and 6%, respectively. Preparation of SC lipid samples and samples with lipophilic moisturizers The isolated CER were mixed with CHOL and FFA or with CHOL and DFFA. The FFA mixture contained C16:0, C18:0, C20:0, C22:0, C23:0, C24:0 and C26:0 in a ratio of 1.3%, 3.3%, 7%, 41.7%, 5.4%, 36.8%, and 4.7% (w/w). This composition is based on the FFA composition reported by Xxxxx and Xxxxxxx (5). Because not all of the FFA are available in a perdeuterated form, the DFFA mixture contained C16:0, C18:0, C20:0, C22:0 and C24:0 in a ratio of 1.3%, 3.3%, 7%, 47%, and 41.4% (w/w). To prepare samples with moisturizer, either perdeuterated or normal moisturizer was dissolved in chloroform/methanol...
Material and Methods. Petitioner shall furnish all material, do all work, and pay all costs for the work described on this Permit.
