Reporters Sample Clauses

Reporters. Commercial banks and U.S. branches and agencies of foreign banks (FR 2028A and FR 2028S only). Estimated annual reporting hours: 7,438 hours. Estimated average hours per response: FR 2028A, 3.65 hours; FR 2028B, 1.4 hours; and FR 2028S, 0.1 hours. Number of respondents: FR 2028A, 398; FR 2028B, 250; and FR 2028S, 567. General description of report: This information collection is authorized by section 11(a)(2) of the Federal Reserve Act (12 U.S.C. 248(a)(2)) and is voluntary. Individual responses 63620 Federal Register / Vol. 76, No. 198 / Thursday, October 13, 2011 / Notices reported on the FR 2028A and FR 2028B are regarded as confidential under the Freedom of Information Act (5 U.S.C. 552(b)(4)).

Reporters. State member banks and their subsidiaries; bank holding companies; savings and loan holding companies; and affiliates of bank holding companies and savings and loan holding institutions, other than banks, savings associations and subsidiaries of banks and savings associations; and nongovernmental entities or persons (NGEPs) that enter into covered agreements with any of the aforementioned companies. Estimated annual reporting hours: Disclosure burden for insured depository institutions (IDI) and affiliates: Covered agreements to public, 6 hours; and Agreements relating to activities of CRA affiliates, 6 hours; Reporting burden for IDI and affiliates: Copy of agreements to agency, 8 hours; List of agreements to agency, 8 hours; Annual report, 8 hours; and Filing NGEP annual report, 6 hours; Disclosure burden for NGEP: Covered agreements to public, 6 hours; Reporting burden for NGEP: Copy of agreements to agency, 6 hours; and Annual report, 24 hours. Estimated average hours per response: Disclosure burden for IDI and affiliates: Covered agreements to public, 1 hour; and Agreements relating to activities of CRA affiliates, 1 hour; Reporting burden for IDI and affiliates: Copy of agreements to agency, 1 hour; List of agreements to agency, 1 hour; Annual report, 4 hours; and Filing NGEP annual report, 1 hour; Disclosure burden for NGEP: Covered agreements to public, 1 hour; Reporting burden for NGEP: Copy of agreements to agency, 1 hour; and Annual report, 4 hours. Number of respondents: Disclosure burden for IDI and affiliates: Covered agreements to public, 2 respondents; and Agreements relating to activities of CRA affiliates, 2 respondents; Reporting burden for IDI and affiliates: Copy of agreements to agency, 2 respondents; List of agreements to agency, 2 respondents; Annual report, 2 respondents; and Filing NGEP annual report, 2 respondents; Disclosure burden for NGEP: Covered agreements to public, 6 respondents; Reporting burden for NGEP: Copy of agreements to agency, 6 respondents; and Annual report, 6 respondents. General description of report: This information collection is mandatory pursuant to Section 48 of the Federal Deposit Insurance Act (12 U.S.C.

Reporters. In Chapter 6 we briefly report on the capacity of schistosome-associated microbiotas to promote B cell and T cell IL-10. The identification of IL-10 producing cell types (especially B cells) is tediously challenging, and often requires several days of artificial ex vivo stimulation followed by flow cytometry staining of IL-10. A variety or transgenic IL-10 reporter lines avoid the need for ex vivo stimulation and intracellular staining, including the IL-10GFP (VeRT-X) strain29. In our hands and others30, we have been able to reliably identify IL-10+ lymphoid cells. However, when using these cell types of care must be taken not to use buffers that will deplete the GFP signal. In addition, these strains are not suitable for the detection of myeloid IL-10 due to autofluorescence coinciding with the GFP emission31. Faecal transplant studies and gnotobiotic approaches The intestinal microbiota is a major calibrator of host physiology and immune development32,33. Both germ free (GF) and antibiotic (Abx)- treated mice allow for the study of set microbiotas or defined bacterial species (and their metabolites) on host responses. In Chapter 4 we evaluate the role of the schistosome-elicited microbiota in skewing of intestinal and mesenteric immune responses through means of faecal transplant into GF animals. Whilst in Chapter 6 we investigate the capacity of defined microbiotas to alleviate allergic airway disease, through antibiotic) mediated depletion of intestinal bacteria, followed by faecal transplant and a model of house dust mite (HDM) evoked allergic inflammation. Experimental design and setup are incredibly important in microbiota studies, as the composition, diversity and metabolic activity of bacterial populations are heavily influenced by external factors34,35. This includes factors such as diet, water, handling of animals, bedding, lighting, and temperature. Although every attention was made to control environmental factors during our investigations, we cannot exclude the possibility that our results our confounded by external influences. The majority of mouse experiments are conducted with specific pathogen free (SPF) mice, whose microbiota is frequently screened for certain pathogens. However, as there is no surveillance or monitoring of commensal organisms, murine microbiotas will vary greatly across institution and different mouse houses will have different definitions of ‘SPF’ mice. When performing experiments with SPF mice, great care must be take...