PAH Sample Clauses

PAH. Ikaria shall transfer to Pulse Technologies (and Pulse Technologies shall accept such transfer) [**] within 30 days after the Effective Date.

PAH. Ikaria shall transfer to Pulse Technologies (and Pulse Technologies shall accept such transfer) the PAH Filings within 120 days after the Effective Date.

PAH. Applies to total PAHs present and includes the following: benzo(a)anthracene, benzo(a)pyrene, benzo(b)fluoranthene, benzo(k)fluoranthene, chrysene, dibenz(a,h)anthracene and indeno(1,2,3-cd)pyrene

PAH. In 1DMW, the catalytic center retains its native state, the ferric ion is chelated by two His, one carboxyl group and three water molecules. There is no direct interaction between the cofactor and the ferric iron. The pterin analog HBI forms hydrogen bonds with those chelating water molecules through the carbonyl oxygen on the pterin ring and this pterin ring π-stacks the phenyl side chain of Phe254. This cofactor also forms directly hydrogen bonds with Gly247, Leu249 and Ala322, and water mediate interactions with His 264 and Glu286. In 4PAH, the crystal structure indicates that a complex between ferric iron and the catechol was formed. Two of the three water molecules which help chelating the ferric iron in 1DMW are replaced by two hydroxyl groups on the catechol group. One of its hydroxyl also forms a hydrogen bond with Tyr325. A cross docking experiment is also performed in this case. Compound HBI can form direct interaction with Fe3+ ion through two hydroxyl group without the solvent molecules, but its polar group will locates in a hydrophobic environment surrounded by Val245, Pro281 and Thr266, which will loose most of its hydrogen bonds with receptor. For ligand LNR, the existence of the water molecules would prevent its direct interaction with metal ion which is the major polar interaction between LNR and receptor (details in Figure 2.13). In this case, solvent molecules play as medium to connect ligand HBI with metal ion in 1DMW. It can be considered as an extension of the ligand, and functionalize as a fragment of the ligand. In this case, they act as part of the ligand and help chelating the metal ion in the binding site. However, these water molecules are not fixed in all crystal structures. In 4PAH, the two hydroxyls on catechol replace two of those three water molecules and directly interact with the ferric. XX0 XX XX X XX XX X XX XX X XX0 XX HBI in 1DMW LNR in 4PAH Figure 2.12 2D structures of HBI (left) and LNR (right).

PAH. XX0+X0 XX0+X0X