Experimental Construction of Plasmids Sample Clauses

Experimental Construction of Plasmids. A sequential PCR method was used to mutate the p420 into the ε containing construct. Primers pISG38 and SKD56 were used to amplify p420; Primers pISG39 and SKD56 were used to amplify that product; and primers pISG40 and SKD56 were used to amplify that product. The final construct was cut with HindIII and KpnI and cloned into pBAV as previously described for ―library 423.‖
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Related to Experimental Construction of Plasmids

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