Unit Definition definition

Unit Definition. One unit of enzyme is defined as the amount that will incorporate l0nmoles of dNTPs into acid insoluble material per 30 minutes at 74 (degrees) C under the analysis conditions below. Analysis conditions: 25mM TAPS (tris-(hydroxymethyl)-methyl-amino- propanesulfonic acid, sodium salt), pH 9.3 (at 25 (degrees) C); 50mM KCl; 2 mM MgCl(2); 1mM (beta)-mercaptoethanol; 200 (micrometer)M each dATP, dGTP, dTTP; 100 (micrometer)M [is proportional to-(32)P]-dCTP (0.05 to 0.1 Ci/mmole); activated salmon sperm DNA, mixed in a final volume of 50 (micrometer)l and incubated at 74 (degrees) C for 10 minutes. The salmon sperm DNA is activated by a modification of the methods in reference 1. The array mixture (without enzyme) is prepared fresh daily and 45 (micrometer)l aliquots (0 (degrees) C) are mixed with 5 (micrometer)l of Taq DNA polymerase diluted in 25mM Tris-HCl pH8, 50mM KCl, 100 (micrometer)g/ml autoclaved gelaxxx, xxX (xxta)-mercaptoethanol, 0.5% w/v) NP40 and 0.5% (w/v) Tween20. Reactions are initiated with addition of enzyme and placed at 74 (degrees) C. Reactions are quenched after 10 minutes with the addition of 10 (micrometer)l of 60mM EDTA and placed at 0 (degrees) C. Aliquots (50 (micrometer)l) are diluted with lml of 2mM EDTA containing 50 (micrometer)g/ml sheared salmon sperm DNA, and precipitated by the addition of 1 ml 20% (w/v) acid and 2% (w/v) sodium pyrophosphate, and incubated at 0 (degrees) C for
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Unit Definition. A unit is a structure where a person(s) may reside. Examples are listed below for reference. Example #1: One apartment building with 32 apartments would be considered as 32 units (32 x $750 = $24,000). If connecting to wastewater service, there would be an additional fee of $24,000 for wastewater. Example #2: Subdividing lots – 6 lots with 4 duplexes (6 x 4 = 24 units x $750 = $18,000) Example #3: Subdividing lots – 10 lots with single family residences – one residence per lot (10 x $750 = $7,500) Note 1: If connecting to water service and wastewater service, $750 must be paid for water and $750 must be paid for wastewater, for a total of $1,500 per unit.
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Unit Definition. One unit of enzyme is defined as the amount that will incorporate 10nmoles of dNTPs into acid insoluble material per 30 minutes at 74 deg. C under the analysis conditions below. Analysis conditions: 25mM TAPS (tris-(hydroxymethyl)-methyl-amino- propane-sulfonic acid, sodium salt) pH 9.3 (at 25 deg. C); 50mM KCl; 2mM MgCl(2); 1mM Beta-mercaptoethanol; 200muM each dATP, dGTP, dTTP; 100muM [infinity - (32)P]-dCTP (0.05 to 0.1 Ci/mmole); activated salmon sperm DNA, mixed in a final volume of 50mul and incubated at 74 deg. C for 10 minutes. The salmon sperm DNA is activated by a modification of the methods in reference 1. The assay mixture (without enzyme) is prepared fresh daily and 45mul aliquots (0 deg. C) are mixed with 5mul of Taq DNA polymerase diluted in 25mM Tris-HCl pH 8, 50mM KCl, 100mug/ml autoclaved gelatin, 1mM Beta-mercaptoethanol, 0.5% (w/v) NP40 and 0.5% (w/v) Tween20. Reactions are initiated with addition of enzyme and placed at 74 deg. C. Reactions are quenched after 10 minutes with the addition of 10mul of 60mM EDTA and placed at 0 deg. C. Aliquots (50mul) are diluted with 1ml of 2mM EDTA containing 50mug/ml sheared salmon sperm DNA, and precipitated by the addition of 1 ml 20% (w/v) trichloroacetic acid and 2% (w/v) sodium pyrophosphate, and incubated at 0 deg. C for 15 min. Precipitated DNA is collected on GF/C filter discs (2.4 cm) and washed extensively with 5% trichloroacetic acid and 2% sodium pyrophosphate (7 x 5 ml), then 95% ethanol (5ml), dried and counted. ------------------------ (1) Xxxxxxxxxx, X.X. 1966. DNA Polymerase from Escherichi coli. in PROCEDURES IN NUCLEIC ACID RESEARCH eds. Xxxxxxx X.X. and Davies, X.X. Xxxxxx & Row, New York. p. 264. Enzyme/PCR Research Products 32 v.2061097 Activity concentration is determined from replicate assays (at least 3) of replicate parallel, serial dilutions (at least 3) that yield 20-90 pMoles dCMP incorporation in the assay. Activated salmon sperm DNA is used at a concentration that provides linear incorporation values with 20-100 mU of enzyme. This DNA concentration usually represents 12.5-20mug/assay.
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Examples of Unit Definition in a sentence

  • ERCOT shall calculate the Planning Reserve Margin (PRM) for each Peak Load Season as follows: PRM s, i = (TOTCAP s, i – FIRMPKLD s, i) / FIRMPKLD s, iThe above variables are defined as follows: Variable Unit Definition PRM s, i % Planning Reserve Margin—The Planning Reserve Margin for the Peak Load Season s for year i.

  • ESACAMT q = LAFF * max(0, RTAML q, p)The above variables are defined as follows: Variable Unit Definition ESACAMT q $ ERCOT System Administration Fee—The ERCOT System Administration fee for each QSE per 15-minute Settlement Interval.

  • The Generation Resource SCADA Splitting Percentage for each Resource within a net metering arrangement for the 15-minute Settlement Interval is calculated as follows: GSPLITPER q, r, gsc, p = GSSPLITSCA r / GSSPLITSCA rThe above variables are defined as follows: Variable Unit Definition GSPLITPER q, r, gsc, p none Generation Resource SCADA Splitting Percentage—The generation allocation percentage for Resource r that is part of a generation site code gsc for the QSE q at Settlement Point p.

  • The total net payments and charges to each QSE for Energy Imbalance Service at all Resource Node Settlement Points for the 15-minute Settlement Interval is calculated as follows: RTEIAMTQSETOT q = RTEIAMT q, pThe above variables are defined as follows: Variable Unit Definition RTEIAMTQSETOT q $ Real-Time Energy Imbalance Amount QSE Total per QSEThe total net payments and charges to QSE q for Real-Time Energy Imbalance Service at all Resource Node Settlement Points for the 15-minute Settlement Interval.

  • A Lecturer track offer letter should follow the template provided by the OFA and will include a description of the position’s duties and the Unit Definition of Privileges (see 4.3).

  • The total of the Standby Payments to each QSE for all RMR Units represented by this QSE for a given hour is calculated as follows: RMRSBAMTQSETOT q = RMRSBAMT q, rThe above variables are defined as follows:Variable Unit Definition RMRSBAMTQSETOT q $ Reliability Must-Run Standby Amount QSE Total per QSEThe total of the Standby Payments to QSE q for all RMR Units represented by this QSE for the hour.

  • The payment for energy for each hour is calculated as follows: RMREAMT q, r = (-1) * (((FIP + RMRCEFA q, r) * RMRSUFQ q, r / RMRH q, r) * RMRALLOCFLAG q, r + (((FIP + RMRCEFA q, r) * RMRHR q, r, i + RMRVCC q, r) * RTMG q, r, i))The above variables are defined as follows: Variable Unit Definition RMREAMT q, r $ Reliability Must-Run Energy Amount per QSE per Resource by hour—The energy payment to QSE q for RMR Unit r, for the hour.

  • The employer must inform employees on fixed-term contracts of the jobs they can apply for in the enterprise so as to ensure that they have the same chance of obtaining permanent employment as others.

  • Secretary of Housing & Urban Development, 817 F.2d 149, 160 (1st Cir.

  • Other = $100/MWh.The above variables are defined as follows: Variable Unit Definition MAXRESPR k $/MWh Maximum Resource Price for source—The highest Maximum Resource Price for the Resources located at the sink Settlement Point k.

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