Sandwich hybridization Sample Clauses

Sandwich hybridization. Sandwich hybridization is a hybridization assay based on the hybridization of a target nucleo- tide (usually RNA) with differentially labeled capture and detector probes (Wicks et al. 1998). The capture probe labeled with e.g. biotin is used to immobilize the target sequence on a solid support and the detection probe is labeled with a detectable marker such as enzymes (Wicks et al. 1998), digoxigenin (DIG) (Rowan et al. 2005, Leskelä et al. 2005, Huhtamella et al. 2007, Thieme et al. 2008), quantum dot labeled probes (Xxxxxxxxxxx et al. 2008), molecular beacons (MB: Zuo et al. 2007, Xxxxxxxxxxx et al. 2008) or tentacle probes (TB: Xxxxxxxxxxx et al. 2008). The use of MBs or TBs as detection probes enables the elimination of wash steps after the final hybridization step thus shortening the procedure time (Zuo et al. 2007, Xxxxxx- field et al. 2008). In addition, helper probes may be used to prevent “incorrect” secondary structures (Rowan et al. 2005, Thieme et al. 2008). Sandwich hybridization can be performed in a liquid format, where the capture probe is utilized for removal of hybridized cells with magnetic beads (Rowan et al. 2005, Leskelä et al. 2005, Huhtamella et al. 2007, Thieme et al. 2008), or in an immobilized format where the capture probe is used to immobilize the tar- get cells into a detection platform (Zuo et al. 2007, Xxxxxxxxxxx et al. 2008). The latter applica- tion has also been utilized in biosensors (e.g. Xxxxxxxx et al. 2004).