Digestion Sample Clauses

Digestion. Restriction endonucleases were used to digest PCR products for genotyping or SSCP. PCR products were estimated to contain DNA at a concentration of 12 ng/ul. HhaI: PCR product 8ul 10 x buffer C 1ul HhaI 1ul 37°C overnight. Deactivation, 95°5 minutes. BsrI: PCR product 21.5ul 10 x buffer IV 2.5ul BsrI 1ul 65°C 2 hours. Not heat deactivated. PstI/PvuII: PCR product 13ul 10 x buffer 3 1.5ul PstI 0.25ul (20 units) PvuII 0.25ul (40 units) 37°C overnight. Not heat deactivated.
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Related to Digestion

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