BEC2 ANTI - IDIOTYPIC MONOCLONAL ANTIBODY Sample Clauses

BEC2 ANTI - IDIOTYPIC MONOCLONAL ANTIBODY. The GD3 ganglioside is reported to be distributed in various tumor cell lines in amounts ranging from 0 percent (colon carcinoma) to 100 percent (metastatic melanoma and small cell lung cancer) (Fuenxxx 0007, Hougxxxx personal communication). An IgG3 monoclonal antibody, R24, which recognizes the human melanoma GD3 ganglioside was raised in syngenic [(C57BL X BALB/c)F1] mice. Additional syngenic mice were immunized with the R24 monoclonal antibody which resulted in the formation of two anti-idiotypic monoclonal antibodies, designated BEC2 and BEC3 (Chapxxx 1991). Both were screened for antibody production in New Zealand white rabbits, which express the GD3 ganglioside on normal tissue (brain, adrenal, thymus, spleen, small bowel) in distributions similar to that seen in humans (Iwamori 1978, 1981; Sekine 1985). Both BEC2 and BEC3 induced anti-mouse and anti-R24 antibodies, but only BEC2 induced anti-GD3 antibodies (Chapxxx 0001). No toxicity's were observed in the rabbits immunized with BEC2 and necropsy results showed no evidence of inflammation within tissues known to express GD3. 11/102 29 [LOGO] The SILVX xxxdy -------------------------------------------------------------------------------- Numerous publications have reported increased immunogenicity, desired serological responses, and mild toxicity's of anti-id MAb in combination with adjuvants (Mittxxxxx 0000, 1992), aluminum salts (Glenny 1926) or aluminum hydroxide-adsorbed vaccine preparations (Aprixxx 0006). Vaccination with BEC2, a murine anti-idiotvpic monoclonal antibody, could evoke specific IgM and IgG against GD3 in animal models (Chapxxx xxx Houghton, 1991). Based on these reports. ImClone Systems Inc., NY, NY sponsored and conducted phase Ib/IIa studies with BEC2 alone and in combination with various adjuvants.
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