Examples of H2O in a sentence
The antigens were retrieved by heating the slides in 10 mM sodium citrate buffer (0.19 g citric acid monohydrate and 1.2 g trisodium citrate dehydrate in 500 ml H2O) and maintaining the temperature just below the boiling point for 6 minutes.
After a brief wash in H2O and PBS, samples were blocked and further processed as described above for the SCCC samples.
After extensive washing, the cells were stained with a filtered 3,3’-diaminobenzidine (DAB) solution (10mg DAB dis- solved in 10 ml PBS and mixed with 10 ml H2O and 10 μl 30% H2O2) under the microscope and immersed in H2O after appearance of the brown colour.
Samples were allowed to cool, washed twice in H2O and incubated in 0.3% H2O2 for 10 minutes.
IRMPD spectra of (UDMH)2H+ and (N2H4)2H+ were recorded on an optically accessible Xxxxxx XxxXxx ETD quadrupole ion trap (QIT) mass spectrometer, at FELIX23 and at Xxxxx State University (WSU).24 The proton-bound dimer ions were generated in an ESI source using solutions acidified with HCl of 10 mM N2H4 or UDMH in H2O at XXXXX and 25 mM N2H4 in H2O or 25 mM UDMH in 50 : 50 methanol : water at WSU.
The virus was stored in reovirus storage buffer (10 mM Tris pH 7.5, 150 mM NaCl, 10mM MgCl2·6 H2O, 5% sucrose) at -80°C.