Dako definition
Examples of Dako in a sentence
A monoclonal antibody for progesterone receptor (PR; clone PgR636, Dako) was used at a dilution of 1:500.
DNA Ploidy Assessment in Tissue Sections Measurement of the DNA ploidy status was per- formed as described previously.24,25 In brief, 7-Km thick paraffin-embedded tissue sections were incubated with primary antibody directed against Ki-67 (clone MIB-1, Dako).
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For detection of macrophages, adipose tissue sections were incu- bated with a CD68-monoclonal antibody (Clone EBM11, Dako, Denmark).
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HOMA-IR, a well-validated measure of insulin resistance will be calculated to estimate insulin resistance.[49] Immunological methods Total IgE will be measured using ELISA with rabbit anti-human IgE antibodies (Dako, Glostrup, Denmark) and goat anti-human IgE biotinylated antibodies (Vector Laboratories, Burlingame, CA, USA) as capture and detection antibodies, as described previously [43].
The slides were washed twice in PBS for 5 minutes each time, and then the monoclonal mouse antibody labelled slides were treated with the second layer rabbit anti-mouse (1:30; DakoCytomation, Inc) in 5 % serum in PBS for 30 minutes whilst the rabbit (anti-C5b-9) slides were treated with the second layer mouse anti-rabbit (1:50; Dako) in 5 % serum in PBS for 30 minutes.
For double staining, antibodies against endothelial cell–specific antigens (CD31, factor VIII; Dako, Carpinteria, CA) against lymphocytes, monocytes, and other inflammatory cells (CD45-LCA; Dako) and bile duct epithelial cells (keratin 18; LUMC, Leiden, The Netherlands) were combined with immunohistochemistry with HLA class I–specific anti- bodies and in situ hybridization with sex chromosomes.
Western blots were stained with EZH2 (Cell Signaling 52465, lot 7, 1:2,000), Myc (Santa ▇▇▇▇ sc-789, lot D1715, 1:1,000) or MTF2 (Protein Tech, 16208-1-AP, lot ▇▇-▇▇▇-▇▇▇▇, 1:2,000) primary antibodies, which were detected using Dako secondary antibodies (P0161, lot 20033538, P0217 20040441).
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