Thesis summary Clause Samples

Thesis summary. The work presented in this thesis attempts to shed some light on the mechanisms of Okazaki fragment maturation, termination, and chromatin replication using the model organism S. cerevisiae. Work in recent years has led to considerable progress in our understanding of the early stages of replication, including initiation and elongation. However, significantly less is known about the events that occur at end of replication, as well as the mechanisms through which replication occurs on chromatin. This is mostly due to difficulties studying these processes in vivo. The complete reconstitution of these processes with purified proteins in vitro will provide a powerful tool for studying how these complicated processes work in vivo. To that end, I adopted a biochemical approach and built upon the previously reported reconstitution of replication initiation and elongation with purified proteins from S. cerevisiae (▇▇▇▇▇▇ et al. 2015; ▇▇▇▇▇▇ et al. 2017). Initially, I developed purification protocols for the proteins involved in Okazaki fragment maturation and identified the minimal set of proteins required for both complete lagging strand synthesis and termination in vitro (Chapter 3). Interestingly, I obtained evidence showing that excess loaded Mcm2-7 double-hexamers are inhibitory to replication on chromatin, and identified a novel pathway for their removal involving Pif1 (Chapter 4). Later, I developed expression strains and purification protocols for the proteins implicated in de novo chromatin assembly: CAF-1, Asf1, Rtt106, Vps75, and Rtt109. I then identified the minimal set of proteins required for this process in vitro: histones, CAF-1 and Asf1 (Chapter 5). Finally, I determined the requirements for Okazaki fragment maturation and termination on chromatin, and established a fully-reconstituted system for studying complete chromatin replication, including both parental histone recycling and de novo chromatin assembly, in vitro (Chapter 6). In addition to providing a powerful tool for future studies of these processes in vitro, this work provides significant insights into replication and chromatin assembly. To our knowledge, this is the first time Pif1 has been implicated in removing loaded Mcm2-7 from DNA. Indeed, it was previously unknown how (or when) excess Mcm2-7 loaded during G1 are removed from DNA. Furthermore, this is the first reported reconstitution of de novo chromatin assembly in its entirety with purified proteins, and the first study to ident...
View Source

Related to Thesis summary

  • Budget Summary Other Sources (Page BudgetSum 2-3 - Acct 7000), must equal Other Uses (BudgetSum 2-3 - Acct. 8000). Estimated Beginning Fund Balance July,1 2020 for all Funds (Cells C3 - K3) (Line must have a number or zero. Do not leave blank.) OK Estimated Activity Fund Beginning Fund Balance July,1 2020 (Cell C83) (Cell must have a number or zero. Do not leave blank.) OK Transfer Among Funds (Funds 10, 20, 40 - Acct 7130 - Cells C29, D29, F29), must equal (Funds 10, 20 & 40 - Acct 8130 - Cells C52, D52, F52). OK Transfer of Interest (Funds 10 thru 90 - Acct 7140 - Cells C30:K30), must equal (Funds 10 thru 60, & 80 - Acct 8140 - Cells C53:H53, J53). OK Transfer to Debt Service to Pay Principal on Capital Leases (Fund 30 - Acct 7400 - Cell E39) must equal (Funds 10, 20 & 60 - Acct 8400 Cells C57:H60). OK Transfer to Debt Service to Pay Interest on Capital Leases (Fund 30 - Acct 7500 - Cell E40) must equal (Funds 10, 20 & 60 - Acct 8500 - Cells C61:H64). OK Transfer to Debt Service Fund to Pay Principal on Revenue Bonds (Fund 30 - Acct 7600 - Cell E41) must equal (Funds 10 & 20 - Acct 8600 - Cells C65:D68). OK Transfer to Debt Service to Pay Interest on Revenue Bonds (Fund 30 - Acct 7700 - Cell E42) must equal (Funds 10 & 20 - Acct 8700 - Cells C69:D72). OK Transfer to Capital Projects Fund (Fund 60 - Acct 7800 - Cell H43) must equal (Fund 10 & 20, Acct 8800 - Cells C73:D76). OK

  • JOB SUMMARY Vouches sample transaction in audit verification assignments and submits findings to supervisor; • Records proceedings of entry and exit conferences; • Collects and analyses data and statistics; • Prepares audit working papers for review by supervisor; • Undertakes any other duties that may be assigned by the Chief Internal Audit Technician.

  • Project Summary The main objective of the LIFE GAIA Sense project is to demonstrate gaiasense, an innovative “Smart Farming” (SF) solution that aims at reducing the consumption of natural resources, as a way to protect the environment and support Circular Economy (CE) models. More specifically, this project will launch 18 demonstrators across Greece, Spain and Portugal covering 9 crops (olives, peach, cotton, pistachio, potato, table tomatoes, industrial tomatoes, grapes, kiwi, walnut) in various terrain and microclimatic conditions. They will demonstrate an innovative method, based on high-end technology, which is suitable for being replicated and will be accessible and affordable to Farmers either as individuals or collectively through Agricultural Cooperatives. Moreover, LIFE GAIA Sense aims to promote resource efficiency practices in SMEs of the agricultural sector and eventually, contribute to the implementation of the Roadmap to a Resource Efficient Europe. This project will demonstrate a method on how the farmer will be able to decide either to use or avoid inputs (irrigation, fertilizers, pesticides etc.) in a most efficient way, without risking the annual production. The focus is on the resource consumption reduction side of CE, and the results will be both qualitatively and quantitatively, considering the resources’ efficiency in agricultural sector.

  • Project Description In two or three brief sentences, provide a concise description of your exhibition. Include the subject matter, type of objects to be included (paintings, sculpture, manuscripts, etc.), those responsible for organizing the exhibition, and catalogue author(s).

  • Overview (a) The Employer is committed to maintaining a stable and skilled workforce, recognising its contribution to the operation of the Employer. As such, full time direct and ongoing employment is a guiding principle of this Agreement. (b) The Employer will take all measures to achieve employment security for the direct permanent employees of the Employer. The Parties agree upon the measures in this Clause to protect and enhance the employment security, health and safety, terms and conditions of employment and career development of the employees. (c) The employer agrees that it is highly important to ensure that work is performed effectively, efficiently and without undue pressure or bullying, and in a way that promotes OHS and EO principles and practices in the workplace and appropriate representation of employees should they so request. The employer will ensure that its employment practices are consistent with the above principles and practices.