Construction and Preparation of the recombinant cosmid Sample Clauses

Construction and Preparation of the recombinant cosmid. A-1. Insertion of a target DNA into the cosmid vector To obtain recombinant adenoviruses, first, an insert DNA is inserted into cos- mid vectors. Both of cosmid vectors contain Smi I (Swa I) and Cla I cloning site, target DNA is usually inserted into Smi I site after the terminal of the target DNA is converted to blunt end. (It is possible to use Cla I cloning site after cutting both the vector and a target DNA with Cla I.)
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Related to Construction and Preparation of the recombinant cosmid

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