Biochemistry reagents Sample Clauses

Biochemistry reagents. Phosphate buffered saline x 1 sterile (PBS) Gibco, Invitrogen Life Technologies) Phosphate buffered saline x 10 sterile (PBS) Gibco, Invitrogen Life Technologies) NHS activated columns (GE healthcare) HiTrap Heparin columns 1ml (GE Healthcare). Glycine (Sigma-Xxxxxxx). Heparan-sulphate from shark cartilage (Sigma Xxxxxxx). Chondroitin sulfate C sodium salt from shark cartilage (Sigma Xxxxxxx). Chondroitin sulfate A sodium salt from bovine trachea (Sigma Xxxxxxx) Calcium chloride (CaCl2) (Sigma Xxxxxxx). Dithiothreitol (DTT) (Sigma Xxxxxxx). Lumitein protein stain (Biotium, UK) NuPage protein gels, Bis-Tris 12%, 1.5mm thickness 10/12 and 15 well formats (Life Technologies). Tween20 (Sigma-Xxxxxxx) Bovine Serum Albumin (BSA) (Sigma-Xxxxxxx). NuPage LDS reducing buffer (Invitrogen Life Technologies). 1-Step Ultra TMB-Blotting Solution (HRP substrate) (Thermo-Xxxxxx, UK) 1-Step Ultra TMB-ELISA Substrate Solution (HRP substrate) (Thermo Xxxxxx, UK) Sodium Chloride (NaCl) (Sigma-Xxxxxxx). Sulphuric acid (Sigma-Xxxxxxx). NuPage MOPS buffer x 20 (Invitrogen, Life Technologies) Bench Mark pre-stained protein ladder (Invitrogen, Life Technologies). Protran nitrocellulose membranes (Whatman, GE Healthcare) Millicoat™ ECM Screening Kit (Millipore)
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