SOLUBILITY TEST. Add 1 mL heptane into a tube containing 2 mL of distilled water. Shake the tube and record your observations. Repeat the test for both cyclohexene and toluene. Test the solubility of these hydrocarbons in each other using 1 mL sample in a clean and dry test tube for each test.
SOLUBILITY TEST. Add 0.5 mL ethanol to a test tube containing 1 mL distilled water. Shake the test tube and record your observation. Repeat the solubility test for 2-butanol, 2-metyl-2-propanol (t-butanol) and phenol. Repeat the test for the solubility of ethanol, 2-butanol, t-butanol and phenol in 1 mL ether and in 1 mL toluene. Record your observations.
SOLUBILITY TEST. Solubility tests were carried out by saturating NADES with an excess of the tested compound at 40 oC, as reported in our former study (Dai et al., 2013). The resulting solution was analyzed with HPLC-UV at a wavelength of 370 nm for quercetin and 517 nm for carthamin. The solubility test was repeated three times.
