Common use of Primary Invader Reaction Clause in Contracts

Primary Invader Reaction. The Institution will design two (2) target-specific oligonucleotide probes (an "Invader" probe and a "Primary" probe) for use in the Primary Invader Reaction. The Invader probe will be designed to hybridize to the 3' portion of the target sequence, and form a region that overlaps the duplex formed by the Primary probe and target by at least a single nucleotide base. The Institution's proprietary Cleavase enzymes will recognize the structure created by this overlapping region and cleave the 5' end of the Primary probe for use in the Secondary Invader Reaction. - The melting temperature (T(m)) of the target-specific Primary probe will be estimated. In order to optimize signal generation, a temperature near the T(m) of the Primary probe will be utilized for the Primary Invader Reaction such that the reaction cycle (hybridization of Primary probe, cleavage of the 5' end of the Primary probe, release of the remaining 3' portion of the Primary probe) will occur rapidly under such conditions. The rapid cycling of the Primary probes allows each copy of mRNA target to serve as the substrate for multiple Primary probe cleavage events during reaction incubation. The accumulation of 5' fragments of the Primary probe is directly proportional to the number of mRNA target present in the test sample.