Media for S. cerevisiae Sample Clauses

Media for S. cerevisiae. In suspension, S. cerevisiae cells were grown in Yeast Peptone (YP) medium (1% yeast extract, 2% peptone) with glucose, raffinose, or galactose to a final concentration of 2%. For growth on solid media, 2% agar was added to YPD. For selective growth on solid media, minimal drop-in media (2% agar, 2% glucose, and 1x yeast nitrogen base) was supplemented with amino acids as required: adenine (5 mg/mL), histidine (10 mg/mL), leucine (10 mg/mL), tryptophan (2 mg/mL), and/or uracil (2 mg/mL).
Sample 1
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