COLLECTION AND HANDLING. Specimen: Serum is the specimen of choice. Plasma collected in heparin may be used. Anticoagulants containing oxalate, citrate or EDTA cannot be used because these substances inhibit the alkaline phosphatase activity.13 Patient Preparation: The patient should be fasting. Patients who have B or O blood group and are secretors may have an elevated ALP about two hours after a fatty meal.6, 12, 13, 18, 19
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