Common use of BACKGROUND AND RATIONALE Clause in Contracts

BACKGROUND AND RATIONALE. sequencing platforms allow sequence-based genotyping — sequencing platforms allow digital expression profiling — (i.e., Genome Sequencer-20 System, Solexa, ABI-based systems) — Sb genome sequence/genetic map alignment allows sequence/SNP mapping — haplotypes or graphical genotypes are revealed at sufficient marker density — graphical genotypes of germplasm will allow better parent/progeny selection — graphical genotyping will accelerate QTL mapping to gene discovery — graphical genotyping may replace targeted marker-assisted-breeding — TAES has developed a graphical genotyping method called Restriction Site Localized sequencing technology (RSL sequencing technology) — TAES has tested RSL-technology on rice/sorghum using 454 technology — RSL-sequencing provides a way to sequence genomes at specific sites — RSL provides a way to re-sequence the same sub-sample of any genome — the number of sites sequenced can be varied depending on need — Solexa can collect 1B bp of sequence per run (25-35bp/read) (~$3,000/run) — sequence sampling every ~4 kbp (two reads/site) = 10 Mbp of sequence — predicted SNP discovery rate = 1 SNP/82kbp (@ 1SNP/1000bp) — indexing allows ~100 genotypes per run (~$30/genotype for sequencing) — more genotypes can be run at lower sequence/SNP coverage — @ $3/~500 marker-genotype this may replace the need for MAB — a high quality genome sequence aligned to a genetic map is required Technical Plan:

BACKGROUND AND RATIONALE. sequencing platforms allow sequence-based genotyping — - sequencing platforms allow digital expression profiling — - (i.e., Genome Sequencer-20 System, Solexa, ABI-based systems) — - Sb genome sequence/genetic map alignment allows sequence/SNP mapping — - haplotypes or graphical genotypes are revealed at sufficient marker density — - graphical genotypes of germplasm will allow better parent/progeny selection — - graphical genotyping will accelerate QTL mapping to gene discovery — graphical genotyping may replace targeted marker-assisted-breeding — Confidential Treatment Requested and the Redacted Material has been separately filed with the Commission - TAES has developed a graphical genotyping method called Restriction Site Localized sequencing technology (RSL sequencing technology) — - TAES has tested RSL-technology on rice/sorghum using 454 technology — - RSL-sequencing provides a way to sequence genomes at specific sites — - RSL provides a way to re-sequence the same sub-sample of any genome — - the number of sites sequenced can be varied depending on need — - Solexa can collect 1B bp of sequence per run (25-35bp/read) (~$3,000/run) — - sequence sampling every ~4 kbp (two reads/site) = 10 Mbp of sequence — - predicted SNP discovery rate = 1 SNP/82kbp (@ 1SNP/1000bp) — - indexing allows ~100 genotypes per run (~$30/genotype for sequencing) — - more genotypes can be run at lower sequence/SNP coverage — - @ $3/~500 marker-genotype this may replace the need for MAB — - a high quality genome sequence aligned to a genetic map is required Technical Plan:: [***]