RPR test definition

RPR test.ย The RPR is a "non-treponemal" test, in that the antibodies detected are not specific for T. pallidum, although their presence in patient's serum or plasma is strongly associated with infection by the infecting organism. These antibodies tend to disappear after successful cure of infection. RPR kits use carbon particles coated with a mixture of lipid antigens, which will combine with antibody present in patient's serum or plasma. Positive reactions are shown by macroscopic aggregation of the particles. ๐Ÿ”พ Equipment required: Micropipette, rotator ๏ฟฝ๏ฟฝ Test procedures: โต Qualitative Test: ๐ŸžŸ The positive and negative kit controls must be run with each batch of tests. ๐ŸžŸ Place 50 ml of specimen or control into a circle on the test card. ๐ŸžŸ Spread the specimen evenly over the test circle area. ๐ŸžŸ Shake the vial of RPR antigen to ensure thorough mixing. ๐ŸžŸ Holding the dropping bottle vertically over the test specimen, dispense a single drop of antigen. ๐ŸžŸ Place test card on a card rotator and rotate at 100 rpm for 8 minutes. ๐ŸžŸ Read and interpret results visually in good light. โต Quantitative Test: ๐ŸžŸ Make doubling dilutions of specimen in normal saline. ๐ŸžŸ Place 50ยตl of each dilution into a separate circle on the test card. ๐ŸžŸ Spread each dilution evenly over the test circle. ๐ŸžŸ Continue as from Qualitative test section 5. The titer of specimen is expressed as the reciprocal of the highest dilution showing aggregation of the carbon particles. ๐Ÿ”พ Interpretation: โต Reactive: Clumps of carbon particles with a clear back ground โต Non-reactive: A smooth grey pattern or a button of non-aggregated carbon particles in the centre of the test circle. โบ TPPA test: TPPA test is very specific for the diagnosis of syphilis. This test is carried out in microwell plate. STI SOP - Sep 07 The antigen, in this TPPA test, is coated on latex particles which are is known as sensitized particles. Antibodies become detectable after 3-4 weeks of infection and may remain detectable for long periods. ๐Ÿ”พ Equipment Required: Micropipette, automatic vibratory shaker, micro-well plate (having โ€œUโ€ shaped bottom). ๐Ÿ”พ Test Procedure: โต Keep 100 ฮผl of diluent (provided in the testing kit) into the well no.1. โต Keep 25 ฮผl of diluent into the well no. 2, 3 and 4. โต Keep 25 ฮผl of patient serum into the well no. 1 and mix thoroughly by filling and discharging by micropipette 4-5 times. Transfer 25 ฮผl of the mixture of specimen and sample diluent into well #2. Then mix well and repeat this procedure again with โ–‡โ–‡โ–‡โ–‡โ–‡ ...
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Examples of RPR test in a sentence

  • One thousand samples were tested, final result indicated that sensitivity of the RPR test and the EIA test are 86.1% and 100%, respectively, and that the specificities are 99.4 and 99.9%o, respectively.

  • West B et al conducted the study in Gambia in 2002 to assess the rapid plasma โ–‡โ–‡โ–‡โ–‡โ–‡โ–‡ (RPR) test performance in the field and to evaluate a new rapid syphilis test (RST) as a primary screen for syphilis 1325 women of reproductive age were tested for syphilis seropositivity using a RPR and a RST strip.

  • The study found that the interpretation of the results of manual techniques, such as the RPR test, the VDRL test, the TPHA assay, and the TPPA assay, can vary greatly among different TSs and operators.

  • Five hundred and ninety five consecutively recruited voluntary blood donors were screened for syphilis using Rapid Plasma โ–‡โ–‡โ–‡โ–‡โ–‡โ–‡ (RPR) test.

  • In addition to the improved sensitivity and specificity of the EIA, other potential benefits of this assay lead to believe that this method could serve as a better screening tool than the RPR test (16).

  • When 6,548 of the EIA- reactive specimens were tested with an RPR test, 2,884 (44%) were reactive and 3,664 (56%) were nonreactive to the RPR test.

  • Sensitivity and specificity of the automated RPR test relative to the TPPA test was 52.5% (95% CI 39.1% to 65.7%) and 94.3% (95% CI 84.3% to 98.8%), respectively, while the same values for the conventional RPR card test were 86.4% (95% CI 75% to 93.9%) and 94.3% (95% CI 84.3% to 98.8%), respectively.

  • The conventional RPR card test showed overall higher positivity than the automated RPR test (24).

  • Their activities mainly involve placement of TST and reading of TST results, provision of routine blood tests for HIV and rapid plasma โ–‡โ–‡โ–‡โ–‡โ–‡โ–‡ (RPR) test for syphilis, patient education, administrative performance checks at the shelters, and provision of incentives to clients to return for TST reading.

  • The RST was easier to use and interpret than the RPR test especially where field conditions were difficult.