R&D Systems definition
Examples of R&D Systems in a sentence
Cathepsins B and L (R&D Systems) and papain (Sigma) were assayed as described previously, using the substrates Z-Leu-Arg-AMC (R&D Systems) for the cathepsins and Z- Phe-Arg-AMC (Bachem) for papain.24 Cloning and Expression of Recombinant Human Vanin-1.
Production of human IL-1b, IL-6, TNFa, IL-10, and IFNg in cell culture supernatants was measured by using human IL-1b, IL-6, TNFa, IL-10, and IFNg enzyme-linked im- munosorbent assay kits (R&D Systems, Minneapolis, MN) following the instructions of the manufacturer.
After washing, streptavidin-HRP conjugate (1:200; R&D Systems) was added.
The serum-free and low-serum cultures were stimulated with different cytokines: recombinant human TNF-α (R&D Systems, Minneapolis, MN USA) at 10ng/ml, recombinant human IL-4 (R&D Systems, Minneapolis, MN USA) at 10 ng/ml, recombinant human IL-13 (R&D Systems, Minneapolis, MN USA) at 10 ng/ml, and anaphylatoxins: human complement proteins C3a and C5a (▇▇▇▇▇▇▇▇▇▇ Industries International, Inc) at a concentration range of 10-10 M to 10-7 M.
After washing the plate with PBS buffer containing 0.05 % Tween-20 (R&D Systems), appropriate dilutions of samples and standards were added, incubated for 2 hours at room temperature, washed, and incubated with a detection antibody for 2 hours at room temperature.
Biochemical Analysis Commercially available enzyme immunoassays (R&D Systems Inc., Abingdon, UK) were used to measure serum concentrations of IL-6 (Sensitivity 0.04 pg.mL-1; CV 7.4%) and TNFα (Sensitivity 0.191 pg.mL-1; CV 8.7%).
A summary of an ELISA protocol is outlined here: A 96-well plate was incubated overnight with the capture antibody at 0.5 µg/ml in PBS (pH 7.20 in sterile water), and subsequently blocked with PBS containing 1 % BSA (R&D Systems).
The reaction was stopped with 2 N H2SO4 (R&D Systems), 50 μl per well.
This Stock Option and Grant Plan (the "Plan") is intended as a performance incentive for officers, employees, directors, consultants and other key persons of R&D Systems Company (the "Company") or its Subsidiaries (as hereinafter defined) to enable the persons to whom options are granted (the "Optionees") or to whom shares of common stock are granted (the "Grantees") to acquire or increase a proprietary interest in the success of the Company.
R&D Systems and the Company shall have entered into a Research Agreement substantially in the form of Exhibit 8 hereto.