FRET definition
Examples of FRET in a sentence
In addition, if Peptide has identified a FRET Substrate for any of the Target Proteases, Peptide will supply Lilly with approximately 10 mg of such Substrate for evaluation by Lilly.
Due to the proximity of these dyes in the uncleaved Signal probe, the fluorescein label is quenched by the quencher dye via a Fluorescence Resonance Energy Transfer (FRET) mechanism.
Cleavage of the 5' end of the Signal probe between the labels enables spacial separation of the quencher dye and fluorescein, thus enabling fluorescence detection using FRET technology.
The generic library contains over [ ]* FRET compounds that are potential protease substrates.
TRF & HTRF® (Time Resolved Fluorescence & Time Resolved FRET) A robust, versatile and sensitive microplate luminometer (lowest crosstalk) exists also in a Clinical version (LB961).
This chapter begins with a description of the physical principles of fluorescence and FRET.
Because MSH2/MSH3 binds with equal apparent affinity to the (CA)4 loop and the (CAG)13 hairpin templates, the kcat/KM is expected to be similar, but the altered recognition properties of MSH2/MSH3 on the low FRET population cannot be resolved in bulk measurements [29, 116].
Under hydrolytic conditions (+Mg), addition of ATP to the (CAG)13-bound MSH2/MSH3 shifted the equilibrium populations towards the low FRET state (Figure 4.7E).
Upon irradiation at 320 nm, a FRET signal will be transferred from the Eu chelate to the ULight fluorophore, which, in turn, generates a light signal at 665 nm.
FRET is most sensitive (the relative FRET change per distance change is the largest) when the donor and acceptor are about R0 apart (R0 is estimated to be ~ 60 Å for Cy3 and Cy5 conjugated to double-stranded DNA), so the most sensitive change in 2 Single-molecule FRET is relatively insensitive to incomplete labeling of a host molecule.