Boehringer Mannheim definition
Examples of Boehringer Mannheim in a sentence
Transfection was performed with FugeneTM6 Transfection Reagent (Boehringer Mannheim, Mannheim, Germany) and selected by 1 mg/ml Hygromycin B (Boehringer Mannheim), as described earlier [Litvinov et al., 1997].
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Cells were transfected using the DOTAP reagent (Boehringer Mannheim Corp., Mannheim, Germany) as described earlier (litvinov et al., 1994a).
The conjugation was performed according to the manufacturer’s protocol (Boehringer Mannheim Corp.).
For brightfield immunohistochemical analysis of axon regeneration rabbit- anti-200-kDa neurofilament (Boehringer Mannheim, Germany; at 1/250) was used as axonal marker.
Cells were rinsed three times with cold PBS, and 2 ml of cold extrac- tion buffer (50 mM Tris/HCl, pH 7.0, 50 mM NaCl, 3 mM MgCl2, 0.5% Triton X-100, 300 mM sucrose, and protease inhibitor [complete; Boehringer Mannheim Corp.]) were added to the cells.
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For extraction, cells were incubated with 0.5% Triton-X-100 in extraction buffer (50 mM TrisHCl, pH7.4, 100 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, 300 mM sucrose, 1 tablet complete protease inhibitor (Boehringer Mannheim), gently scraped from the dish, collected and rotated for 30 minutes at 4˚C.
Briefly, cells were dissociated with TC, washed in Dulbecco’s PBS, labeled with one of the fluorochro- mes, washed three times in 50% FCS in DME, resuspended in DME containing 0.8% FCS and 1 mg/ml DNAse (Boehringer Mannheim Corp.), mixed at various ratios depending on the experiment, and allowed to aggregate as described for the aggregation assays.
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