Common Contracts
1 similar null contracts
Collaboration type Licence agreement Research cooperationFebruary 1st, 2018
Typically, production process of peptides or proteins of interest by host cells, or mole- cular cloning, requires the use of one or more replicon sequence(s), such as plasmids carrying one or more gene(s) encoding the protein(s) of interest. Cells in bioreactor usually present a high heterogeneity. It has been demonstrated that the growth of plasmid bearing cells, especially bacterial cells, is significantly reduced compared to plasmid free host cells. Indeed, peptide or protein production coming from gene of interest represents a significant burden on the metabolism of the cell. Therefore, it is necessary to select cells that keep the gene of interest. This selection is efficient- ly done by the use of a selective tool, such as antibiotic resistance gene or pair of poison and/or antidote genes. However, classical systems do not prevent frameshift from translation, truncated versions of the protein of interest... which could lead to a contamination by mutated proteins.
