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Licence agreement concerning inclusion of doctoralAugust 20th, 2006
Using pure trans-isocohumulone, cis-isocohumulone, trans-isohumulone, cis- isohumulone, trans-isoadhumulone, and cis-isoadhumulone an isocratic HPLC system has been developed for quantitation of these compounds in hop extracts and beers. The mobile phase contained acetonitrile-water-H3PO4 (50:50:0.01, v/v/v) and was used with a Phenomenex Hypersil 5 µ C18 column 250 x 4.6 mm, flow rate 1.5 ml/minute. Baseline separation of all 6 isomers was achieved with a total run time of 25 minutes. The UV spectrum of these pure compounds using this system were quite different. The effect of different mobile phase compositions on separation was investigated as well as the chromatographic parameters, detection limit, and linearity.
