THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
LOS ALAMOS NATIONAL LABORATORY
WORK FOR OTHERS/FUNDS-IN AGREEMENT NO. FIA-98-053-A001
AMENDMENT NO. 1
This Amendment is by and between The Regents of the University of California,
acting through the undersigned Contracting officer of The Los Alamos National
Laboratory (hereinafter called "LANL") and Centrex, Inc. (hereinafter called the
"Sponsor").
WITNESSETH THAT:
WHEREAS, the parties desire to amend Work for Others/Funds-In Agreement No.
FIA-98-053 by increasing the scope of work; extending the estimated performance
period; and modifying the total estimated cost; and
WHEREAS, the Agreement is authorized and executed under the authority of LANL
NOW THEREFORE, the parties hereto agree as follows:
1. Appendix A, Statement of Work is revised to include the attached
Appendix A, Statement of Work, which defines the increase in scope
resulting from this Amendment No. 1, and which is hereby incorporated
by reference.
2. Pursuant to Terms and Conditions, Section II, Article 2, Term of the
Agreement, the estimated Period of Performance is continued through
July 03, 2003.
3. The original Work for Others/Funds-In Agreement Total Estimated Cost
was $410,000.00. The original Total Estimated Cost is revised to read:
$279,000.00. Work was stopped due to unavailability of Sponsor funding
to continue work under the original Statement of Work.
4. The estimated cost of work for this Amendment No. 1 is $640,226.00, and
the total estimated cost under this Agreement is revised from
$279,000.00 to $919,226.00.
4. Except as herein provided, all other terms and conditions of the Work
for Others/Funds-In Agreement No. FIA-98-053 shall remain unchanged and
in full force and effect.
IN WITNESS WHEREOF, the parties hereto have caused this Amendment No. 1 to be
executed by their duly authorized representatives.
-------------------------------------------------------------- ------------------------------------------------------------
CENTREX, INC. FOR THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
-------------------------------------------------------------- ------------------------------------------------------------
-------------- ----------------------------------------------- ----------- ------------------------------------------------
Name: Xx. Xxxxxx X. Xxxxxxxx, Xx. Name: Xxxxxx .J Xxxxxx
Title: Medical Director Title: Acting Program Manager
Address: 0000 X. Xxxx Xxxxxx, #000 Address: Los Alamos National Laboratory Industrial
Tulsa OK 74137-0000 Business Development Program Xxxxxx
X. X.
Xxx
0000,
XX
X000
Xxx
Xxxxxx,
XX
00000
------------------------------------ ------------------------- ------------------------------ -----------------------------
Telephone: (000) 000-0000 Fax: (000) 000-0000 Telephone: (000) 000-0000 Fax: (000) 000-0000
------------------------------------ ------------------------- ------------------------------ -----------------------------
Signature Date Signature Date
/s/ Xxxxxxx X. Xxxxxxxx, Xx. November 13, 2002 /s/ Xxxx Xxxxxx January 25, 2002
------------------------------------------- ------------------ ------------------------------------------ -----------------
APPENDIX A
Statement of Work
FIA-98-053-A01
Centrex, Inc.
Prototype System for Detection of Bacterial Contamination
I. Description of Services
1.0 Objective
The focus of this project will shift towards the detection and
quantitation of bacterial and viral biowarfare agents in
environmental samples. The instrument prototype will have the
additional ability of detecting 5 to 10 different bioagents.
The pathogens to be identified as targets will include some or
all of the following: Bacillus anthracis, Yersinia pestis,
Variola major, Francisella tularensis, Escherichia coli
O157:H7, and the various viral hemorrhagic fever agents. These
bioagents will be specified during the project, depending on
the availability of specific sequences for each pathogen, and
the identification of newly emerging threats by the
appropriate goverment agencies.
2.0 Scope
Phase I will be extended to include:
i. Development of DNA extraction protocols from liquid
suspension samples. ii. Development of the polymerase
extension protocol for each of the pathogens to be analyzed.
Phase II will be extended to include:
i. Construction of an automated DNA extraction robotic system.
Our goal is that the entire process can be automated beyond
the introduction of the sample.
The project will take an estimated 18 months from the date
that work commences under FIA-98-053-A001. The technical tasks
table below shows the additional tasks that need to be
performed for completion of the project and their respective
durations.
3.0 Applicable Documents
No Changes Under 3.0 Applicable Documents from Original
Statement of Work.
4.0 Technical Tasks
Phase Task Description Start Duration
Month (Months)
I. Development of additional molecular biology protocols. 18 Months
Total
Estimated
1. Identification of specific DNA sequences for each Duration
pathogen. Through
Possible candidates sequences to be used with the Estimated
polymerase extension protocol will be obtained from a End Date of
variety of sources. Best sequences will be selected July 03,
using computational methods and success of related 2003
techniques (e.g. PCR).
2. Demonstration of the polymerase
extension protocol for each pathogen.
The protocols developed during the
original project will be adapted to
each of the pathogens to be analyzed.
Synthesis of fluorescent reporter
molecules will be demonstrated.
4.0 Technical Tasks (continued)
3. Development of DNA extraction
protocols. These protocols will be
demonstrated using liquid suspensions
of non-pathogenic surrogates (either
Bacillus globigii spores,
non-pathogenic Bacillus anthracis or
non-pathogenic E.coli).
II. Construction of optical subsystem.
The high-collection-efficiency fluorescence detection
optical subsystem will be constructed. This subsystem
consists of excitation components (laser, focusing
optics), fluorescence collection optical components,
spectral and spatial discrimination components, and
single-photon detection components, in accordance to
established single-molecule detection technology.
1. Construction of electronics subsystem.
This subsystem comprises the signal
amplification and conditioning
electronics, scaling electronics,
computer interface electronics, and
power supplies for various electronic
components.
2. Construction of DNA extraction and
sample delivery subsystem. The
automated nucleic acid extraction and
injection subsystem will be
constructed. It consists of a robotic
system for DNA extraction and enzymatic
reaction set-up, and components for
incubation, fluorescence micro-cell and
sample injection.
3. Implementation of data acquisition and
automated control subsystem. In this
stage, the data acquisition and
automated control software will be
written in the LabVIEW programming
environment.
4. Final system integration and test. The electronics,
optical, and sample handling subsystems will be
incorporated into an instrument chassis at this
point. Complete prototype operation will be
demonstrated with non-pathogenic surrogates (either
Bacillus globigii spores, non-pathogenic Bacillus
anthracis or non-pathogenic E.coli). The limits of
detection of the instrument will be determined by
detecting increasingly smaller quantities of
synthetic forms of each pathogen's DNA.
II. Reports, Data, and Other Deliverables
i. A prototype instrument for the detection of biological warfare
pathogens. ii. Quarterly reports. iii. Test results.iv. Complete
prototype specifications.
III. Special Considerations
No Changes to Special Considerations from Original Statement of Work.
IV. Designated Signature Authorities/Representatives
Centrex, Inc. Los Alamos National Laboratory
Attn.: Xx. Xxxxxx X. Xxxxxxxx, Xx. Attn.: Xxxxxxx X. Xxxxxxx
Medical Director Agreements Contract Specialist
Industrial Business Development
Program Xxxxxx
0000 X. Xxxx Xxxxxx, #000 Xxx Xxxxxx National Laboratory
Xxxxx, XX 00000-0000 P. O. Xxx 0000, XX X000
Tel: (000) 000-0000 Xxx Xxxxxx, XX 00000
Fax: (000) 000-0000 Tel: (000) 000-0000
Fax: (000) 000-0000
