Retina collection for HPLC detection of dopamine Sample Clauses

Retina collection for HPLC detection of dopamine. Following light and lens defocus treatment animals were sacrificed. eyes were enucleated, and retinas collected (control: n=15/light level, LIM: n=16/light level). Retinas were immediately frozen on dry ice and kept in storage at -80° C. Collected retinas from both eyes of LIM and control mice were analyzed by high performance liquid chromatography (HPLC) to determine levels of DA and its metabolite 3,4- dihydroxyphenylacetic acid (DOPAC). First, to measure DA and DOPAC, a previously described analysis method was used (Nir, 2000; Xxxxxxxx et al., 2008). Briefly, retinas were homogenized in 0.1 N perchloric acid, 0.01% sodium metabisulfite with 25 ng/ml 3,4- dihydroxybenzylamine and centrifuged. Supernatant was injected into a Xxxxxxx Ultrasphere 5µm ODS column, 250 x 4.6 mm (Fullerton, CA). The mobile phase consisted of 0.1M
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