Pfizer Deliverables Sample Clauses

Pfizer Deliverables. Pfizer will deliver to Xenogen, a sterile preparation of *** of ready-to-transfect DNA for *** targeting vectors along with *** of single-copy verified DNA for generating Southern probes from regions contained in the *** and from the *** for each of the *** targeting vectors. The targeting vectors will be delivered at the rate of approximately *** per month. The targeting vectors will be ***. All vectors will include at least ***. Each targeting vector shipment will be accompanied with supporting sequence information for the gene locus, the targeting vector, relevant restriction maps, restriction enzyme data, ▇▇▇▇▇▇▇▇ ▇▇▇▇ confirming the structure of the targeting locus and the targeting vector. Each targeting vector will be accompanied with a Southern screening strategy using *** for each of the *** targeting vectors. All information pertaining to these projects, including the gene identities, gene sequence information, and screening strategies, is Pfizer confidential information and should be treated by Xenogen as such. Pfizer will deliver to Xenogen, Pfizer’s *** cell line along with *** cell line specific culturing protocols, reagents information and any training necessary to become familiar with this cell line. Pfizer will provide vials of mycoplasma and MAP tested *** cell line on an as-need basis to support transfection of the *** targeting vectors and any repeat transfections. Initially Pfizer will send *** vials of the said cell line to Xenogen. Xenogen’s role will be, for each targeting vector, to carry out all necessary embryonic stem cell manipulations, blastocyst injection, and animal husbandry to deliver germline chimeric mice from a single targeted clone for at least *** out of the *** targeting vectors Karyotype analysis should be performed by the ▇▇▇▇▇▇▇ Institute (Camden, NJ) on each gene-targeted clone, and a normal karyotype ensured before blastocyst injection is initiated. In the event that a cell line is karyotyped as normal by ▇▇▇▇▇▇▇, and that cell line is used to produce *** but no germline chimeras are produced, the cell line in question will be counted towards the *** required projects that deliver germline chimeras. In addition, for each targeting vector, the available germline chimeras will be bred as necessary to deliver *** KO homozygous animals, along with *** wild type (non-littermate) *** controls. No penalty should occur to Xenogen for those projects where embryonic lethality precludes the delivery of homozygous KO mice. ...