Genotyping Method Clause Samples

Genotyping Method. Blood samples were collected at the local institute and sent to the DNA bank at regular intervals for processing to allow analyses of deoxyribonucleic acid (DNA), ribonucleic acid (RNA), protein and immortalised B cells. DNA purification and genotyping was performed by the Centre National de Génotypage in Paris. DNA was purified from whole blood samples (~10ml) preserved in BD Vacutainer EDTA tubes (Becton, ▇▇▇▇▇▇▇▇▇ and Company) using Gentra Puregene Blood Kit (Qiagen) according to the manufacturer’s instructions. Genotype information was collected at 582,982 markers using the Illumina HumanHap610 Genotyping BeadChip. Single nucleotide polymorphisms (SNPs) with call rates of < 98%, minor allele frequency < 1% or deviation from the ▇▇▇▇▇-▇▇▇▇▇▇▇▇ equilibrium (p ≤ 1×10- 4) were excluded from the analyses. Individuals with an ambiguous sex code, excessive missing genotypes (failure rate > 2%), and outlying heterozygosity (heterozygosity rate 3 standard deviations from the mean) were also excluded. Identity-by-state similarity was used to estimate cryptic relatedness for individual using PLINK software. Closely related individuals with Identity-by-descent (IBD > 0.1875) were eliminated from the subsequent analysis. Population stratification for the GWAS data was examined by principal component analysis (PCA) using EIGENSTRAT software. The four HapMap populations were used as reference groups in the PCA and individuals with divergent ancestry (from CEU) were also excluded.
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Genotyping Method. Genotyping will be accomplished by [***] specifically [***] the [***] for each [***]. All [***] from an [***] will be [***] with [***] and [***], and then [***] with [***]. A [***] will then be [***], the [***], and a [***]. The [***] a [***] to the [***] of the [***]. [***] subsequently [***] the [***] after [***] within the [***]. The [***] will be [***] by the [***] and [***] as [***]; each [***] a [***]. [***] of [***] will [***] a [***], while [***] of [***] will [***] a [***]. [***] will be [***] a [***] that is [***] as a [***]. A [***] and a [***] are also [***] with [***] of [***] as a [***] of [***].
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Related to Genotyping Method

  • Billing Method 2.6.1 To receive payment for services rendered pursuant to this contract the Contractor shall submit a fully completed invoice for work previously performed to: 2.6.2 At a minimum, the invoice shall detail the following information: 2.6.2.1 Unique invoice number; 2.6.2.2 Contractor’s name, address, and telephone number; 2.6.2.3 Date of invoice and/or billing period; 2.6.2.4 Applicable Contract No.;

  • Measurement method An isolation resistance test instrument is connected between the live parts and the electrical chassis. The isolation resistance is subsequently measured by applying a DC voltage at least half of the working voltage of the high voltage bus. If the system has several voltage ranges (e.g. because of boost converter) in conductively connected circuit and some of the components cannot withstand the working voltage of the entire circuit, the isolation resistance between those components and the electrical chassis can be measured separately by applying at least half of their own working voltage with those components disconnected.

  • Voting Method Unless otherwise provided by law, when a vote is taken upon any measure before the Board, with a quorum being present, a majority of the votes cast shall determine its outcome. A vote of "abstain" or "present," or a vote other than "yea" or "nay," or a failure to vote, is counted for the purposes of determining whether a quorum is present. A vote of "abstain" or "present," or a vote other than "yea" or "nay," or a failure to vote, however, is not counted in determining whether a measure has been passed by the Board, unless otherwise stated in law. The sequence for casting votes is rotated. On all questions involving the expenditure of money and on all questions involving the closing of a meeting to the public, a roll call vote shall be taken and entered in the Board's minutes. An individual Board member may request that a roll call vote be taken on any other matter; the President or other presiding officer may approve or deny the request but a denial is subject to being overturned by a majority vote of the members present. Any Board member may request that his or her vote be changed before the President announces the result. Any Board member may include a written explanation of his or her vote in the District file containing individual Board member statements; the explanation will not be part of the minutes. The Board Secretary shall keep written minutes of all Board meetings (whether open or closed), which shall be signed by the President and the Secretary. The minutes include:

  • Test method This test is intended to demonstrate the immunity of the vehicle electronic systems. The vehicle shall be subject to electromagnetic fields as described in this annex. The vehicle shall be monitored during the tests. If not otherwise stated in this annex the test shall be performed according to ISO 11451-2.

  • Accounting Method For both financial and tax reporting purposes, the books and records of the Company shall be kept on the accrual method of accounting applied in a consistent manner and shall reflect all Company transactions and be appropriate and adequate for the Company’s business.