Correlative optical and FIB Sample Clauses

Correlative optical and FIB. SEM synaptic maps: EM blocks scans and volume analysis We have generated six synaptic maps comprising FIB/SEM and confocal images (three of the hippocampus, three of the somatosensory cortex); that is three times more than was planned to perform by M30. This dataset is unique in that the volume of tissue that have been reconstructed at the EM level from the adult mouse hippocampus and somatosensory cortex is quite small in existing databases (see for example, Xxxxxx et al., Sci Data; 2:150046. doi: 10.1038/sdata.2015.46. eCollection 2015). Furthermore, high-quality synaptic optical maps of synaptic proteins are sparse. The technology is now available to be exploited for comprehensive studies and generation of high resolution optical synaptic protein maps and 3D reconstruction using automatic EM techniques. However, data on optical synaptic protein maps and reconstructions at the EM synaptic resolution are sparse (see for example Kasthuri et al. Cell 162, 648–661, 2015).
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