Biological Sampling Clause Samples
Biological Sampling. Underwater biological sampling of the marine hull fouling on the Obsolete Vessel is not required and shall not be performed by the Buyer unless authorized by the Contracting Officer in writing. If MARAD determines there is a requirement for conducting biological sampling after the award of the sales contract for the recycling of the vessel the cost for the performance of the sampling activity will be contracted separately by the Contracting Officer.
Biological Sampling i. Check for presence of tags.
Biological Sampling. For each subject, sampling sessions included whole blood collection, passive saliva and exhaled breath condensate collection [41]. Briefly, venous blood was collected in 3mL, ethylenediaminetetraacetic acid-treated (EDTA) tubes (BD Vacutainer) from either the left or right median cubital vein and centrifuged immediately for plasma retrieval. Saliva was collected passively into sterile 15mL conical centrifuge tubes (Falcon) held against the lips. Rtubes (Respiratory Research) were used to collect roughly ~2mL of exhaled breath condensate. Biological samples from each matrix was aliquoted into microcentrifuge tubes and stored at -80⁰C within 20 minutes of collection. Metabolomic analyses were conducted within four months of collection. Metabolomics and Data Processing. Analytical chemistry was performed following procedures developed by ▇▇▇▇▇▇ et al. (2013). For each sample, 65µL were diluted into 130µL of acetonitrile spiked with isotopic standards. After a 30-minute equilibration and protein precipitation, triplicate 10µL aliquots were placed into autoinjector vials and run across two batches through a C18 reverse phase liquid chromatography coupled with high resolution mass spectrometry (HR-MS) on a QExactive Orbitrap (ThermoFisher) [43]. The analytical platform