Acute studies Clause Samples

Acute studies. To confirm the optimal BH parameter space identified ex vivo in Aim 2, we will first treat n=2 intact male dogs with transrectal BH across the parameter space identified in aim 2 to identify the optimum parameter space for in vivo work. We will then perform an initial acute study in which n=5 dogs are treated with transrectal BH targeting a small volume of adenomatous (BPH) prostate (1-2 cc) and then euthanized immediately following treatment to assess/confirm histologic evidence of the efficacy of BH treatment, to assess the accuracy of targeting, to evaluate thermal effects, and to identify any evidence of collateral damage. The rectal wall, prostate, urethra, bladder and adjacent structures will be examined for signs of acute damage. NADH oxidase testing will be used to assess for thermal effects. The treated area will be resected, fixed, paraffin-embedded, sectioned, and stained for histological analysis.