Examples of Hot Start in a sentence
Reactions can also be optimized using the provided DMSO or MgCl2 solutions.Note: Phusion Hot Start Flex DNA Polymerase is unlike other enzymes and care must be taken when designing cycling protocols.
Reaction Setup: Phusion Hot Start Flex DNA Poly- merase is inhibited at room temperature, allowing flexible reaction set up (RT or ice).
We generally recommend using Phusion Hot Start Flex DNA Polymerase at a concentra- tion of 20 units/ml (1.0 unit/50 µl reaction).
However, the optimal concentration of Phusion Hot Start Flex DNA Polymerase may vary from 10–40 units/ml (0.5–2 units/50 µl reaction) depending on amplicon length and difficulty.
The final concentration of each primer in a PCR experiment using Phusion Hot Start Flex DNA Polymerase may be 0.2–1 µM, while 0.5 µM is strongly recommended.