Sequencing Sample Clauses
Sequencing. For the avoidance of doubt, the Board may approve and adopt any matter referred to herein that also requires approval of the Company’s stockholders under the Delaware General Corporation Law prior to the Company obtaining the approval required herein; provided, that the Company may not permit such matter to occur until the approval required herein is obtained.
Sequencing. 1.5.1. Sequence TAB work in conjunction with work by the Contractor. TAB work and milestones shall be incorporated in the General Contractor’s Construction Schedule.
1.5.2. Sequence TAB work to commence after completion of systems. TAB work shall be completed as a prerequisite for Substantial Completion of the Project.
Sequencing. As stated in Section 5, it is expressly understood, agreed and expected that the Closing will be completed before Buyer has had an opportunity to wire the Purchase Price to Seller, but that nonetheless upon the applicable Closing all title and ownership to and in the applicable Livestock shall transfer to Buyer.
Sequencing. It is expressly understood, agreed and expected that the applicable Closing might be completed before Seller has had an opportunity to present the Satisfaction Documents to the Issuing Bank and thereby receive the Purchase Price from the Issuing Bank, but that nonetheless upon the applicable Closing (i.e., upon satisfaction or express waiver in writing of all the closing conditions as set forth herein) all title and ownership to and in the applicable Shipment Gold (i.e., including the refined gold resulting from refining the 50-100 kilogram sample of Gold selected by Buyer, or the dip samples (as the case may be), and also including the remaining Gold in doré bar or other form and all other minerals contained in the doré) shall automatically transfer to Buyer – and without any requirement for further instrument of transfer on the part of Seller.
Sequencing. “Sequencing” as used herein denotes the process performed by a Sequencing Lab, as defined herein, to determine the order of nucleotides from a Sample, as defined herein.
Sequencing. Investments will be financed by the public sector. Their sequencing has been designed to enable: (i) AC-IWRM to be exposed to international best practices for IWRM and to adapt these to local conditions prior to implementation; (ii) the selection of a medium irrigation subproject to rapidly demonstrate principals of IWRM and show quick results in improved water resources management and efficiency, WUCS strengthening and improved agricultural practices; (iii) progressive capacity development of AC-IWRM, KNNL, CADA and WUCS; and (iv) testing and innovation, with a feedback mechanism to incorporate lessons learned into future tranches.
Sequencing. Commented [CS15]: A request was made that this language be reviewed again to make sure it is consistent with the sequencing included in the MDP.
Sequencing. Sema4 shall use [***] to complete the [***] Sequencing of all Biospecimens within [***] months from the date that ISMMS provides all of the Biospecimens to Sema4, as described in Exhibit E. Pursuant to SEC Release 34-85381, certain identified information has been excluded from this Exhibit because it is (i) not material and (ii) would be competitively harmful if publicly disclosed. 3
Sequencing. The sequencing order for development and execution of a cooperative approach under A6.2 is outlined in the diagram below.
Sequencing. The exons, 5’ and 3’ UTRs, and flanking sequences 1kb upstream of CHRNA5, CHRNA3, and CHRNB4 were sequenced. Sequence for the region was obtained from NCBI build 36. A total of 57 primer pairs were designed (see Table 3.10). The position of regions sequenced (build 36) can be found in Table 3.11. PCR amplification and sequencing reactions were set up on Zymark ALH300 workstations, with amplification performed on MJR Tetrads. PCR products were purified using AMPure (Agencourt Bioscience). Dye terminator removal was performed using CleanSEQ (Agencourt) to repurify. Electrophoresis was performed on Applied Biosystems 3730 DNA Analyzers. Sequence editing and analysis were performed using deCODE Genetics Sequence Miner software. SNP calling was done by both manual inspection and automated calling. All SNPs identified through automated calling were then confirmed by manual inspection of the sequence traces. Insertion/deletions and microsatellites were identified by manual inspection. Simple, rare insertion/deletions were called manually. Additional genotyping of SNPs was done using the Centaurus platform (Nanogen). Three variants, rs55787222, rs3841324, and rs60706203, observed in the sequencing, were genotyped in a larger population. For these markers primers were designed using Primer3. PCR reactions were set up on Zymark ALH300 workstations and amplification performed on MJR ▇▇▇▇▇▇▇. PCR products were pooled, an internal size standard added, and then resolved on Applied Biosystems 3730 DNA Analyzers. Primers and PCR conditions are available on request. Genotypes were called and edited using deCODE Allele Caller and deCODE-GT. The variant rs3841324 was identifed as a promoter element with significant effect on transcription of CHRNA5 in a genome scan for regulatory elements(99). We therefore examined its role in regulating expression of the gene in blood and subcutaneous adipose tissue using an expression cohort previously described(100). From this cohort, genotype and expression data were used from 446 individuals with blood samples and 376 individuals with subcutaneous adipose tissue samples. RNA samples were purified using RNeasy Mini Kit (Quiagen), and integrity analyzed using Agilent 2100 Bioanalyzer. Total RNA was converted to cDNA using the High Capacity cDNA Archive Kit (Applied Biosystems). Two Taqman assays were designed for CHRNA5, so that positive results cannot be attributable to the specific assay used. The probes are located at different exon bo...