Common use of Secondary Invader Reaction Clause in Contracts

Secondary Invader Reaction. The Institution will design oligonucleotides that will function as a Secondary target and a "Signal" probe in the Secondary Invader Reaction. The cleaved 5' end of the Primary probe from the Primary Invader Reaction is designed to act as a "Secondary Invader" probe in the Secondary Invader Reaction (i.e., the Signal probe will be designed such that the 3' end of the Secondary Invader probe (5' of the Primary probe from the Primary Invader Reaction) will overlap the 5' most region of hybridization of the Signal probe/Secondary target complex). The Institution's proprietary Cleavase enzymes will recognize the structure created by this overlapping region and cleave the 5' end of the Signal probe for subsequent detection. The Signal probe will include a quencher dye and a fluorescein-phosoramadite label. Due to the proximity of these dyes in the uncleaved Signal probe, the fluorescein label is quenched by the quencher dye via a Fluorescence Resonance Energy Transfer (FRET) mechanism. Cleavage of the 5' end of the Signal probe between the labels enables spacial separation of the quencher dye and fluorescein, thus enabling fluorescence detection using FRET technology. - The melting temperature (T(m)) of the Signal probe will be estimated. In order to optimize signal generation, a temperature near the T(m) of the Signal probe will be utilized for the Secondary Invader Reaction such that the reaction cycle (hybridization of Signal probe, cleavage of the 5' end of the Signal probe, release of the remaining 3' portion of the Signal probe) will occur rapidly under such conditions. This rapid cycling allows multiple Signal probes to be cleaved during reaction

Secondary Invader Reaction. The Institution o TWT will design oligonucleotides an oligonucleotide that will function as both a Secondary target and a "Secondary Signal" probe in the Secondary Invader Reaction. The cleaved After cleavage in the Primary Invader Reaction the 5' end of the Primary probe wild-type and mutant Signal probes from the Primary Invader Reaction is will be designed to act as a "Secondary Invader" probe probes in the Secondary Invader Reaction (i.e., the Secondary Signal probe will be designed such that the 3' end of the Secondary Invader probe (5' of the Primary Signal probe from the Primary Invader Reaction) will overlap the 5' most region of hybridization of the Secondary Signal probe/Secondary target complex). The InstitutionTWT's proprietary Cleavase enzymes will recognize the structure created by this overlapping region and cleave the 5' end of the Signal probe for subsequent detection. The Secondary Signal probe will be designed to include a quencher dye and a fluorescein-fluorescein phosoramadite label. Due to the proximity of these dyes in the uncleaved Secondary Signal probe, the fluorescein label is quenched by the quencher dye via a Fluorescence Resonance Energy Transfer (FRET) mechanism. Cleavage of the 5' end of the Secondary Signal probe between the labels enables spacial spatial separation of the quencher dye and fluorescein, thus enabling fluorescence detection using FRET technology. - o The melting temperature (T(m)) Tm of the Signal Secondary Invader probe will be estimated. In estimated and sequence optimized in order to optimize signal generation, a temperature near the T(m) Tm of the Signal Secondary Invader probe will be utilized for the Secondary Invader Reaction reaction such that the reaction cycle (hybridization of Signal Secondary Invader probe, cleavage of the 5' end of the Signal probe, release of the remaining 3' portion of the Signal Secondary Invader probe) will occur rapidly under such isothermal conditions. This As a result of the Secondary Signal/Secondary target complex being present in excess and the rapid isothermal cycling allows of the Secondary Invader probe allowing each copy of Secondary Invader probe to cause multiple signal generation cleavage events during reaction incubation, the accumulation of 5' fragments of the Secondary Signal probes probe is directly proportional to be cleaved during reactionthe number of Secondary Invader probe molecules generated in the Primary Invader Reaction.

Secondary Invader Reaction. The Institution o TWT will design oligonucleotides that will function as a Secondary target and a "Signal" probe in the Secondary Invader Reaction. The cleaved 5' end of the Primary probe from the Primary Invader Reaction is designed to act as a "Secondary Invader" probe in the Secondary Invader Reaction (i.e., the Signal probe will be designed such that the 3' end of the Secondary Invader probe (5' of the Primary probe from the Primary Invader Reaction) will overlap the 5' most region of hybridization of the Signal probe/Secondary target complex). The InstitutionTWT's proprietary Cleavase enzymes will recognize the structure created by this overlapping region and cleave the 5' end of the Signal probe for subsequent detection. The Signal probe will include a quencher dye and a fluorescein-phosoramadite label. Due to the proximity of these dyes in the uncleaved Signal probe, the fluorescein label is quenched by the quencher dye via a Fluorescence Resonance Energy Transfer (FRET) mechanism. Cleavage of the 5' end of the Signal probe between the labels enables spacial spatial separation of the quencher dye and fluorescein, thus enabling fluorescence detection using FRET technology. - o The melting temperature (T(m)) Tm of the Signal probe will be estimated. In order to optimize signal generation, a temperature near the T(m) Tm of the Signal probe will be utilized for the Secondary Invader Reaction such that the reaction cycle (hybridization of Signal probe, cleavage of the 5' end of the Signal probe, release of the remaining 3' portion of the Signal probe) will occur rapidly under such conditions. This rapid cycling allows multiple Signal probes to be cleaved during reaction.