In Vitro DNA Replication Assays Sample Clauses

In Vitro DNA Replication Assays. 2.14.1 5’-32P-labelling of HindIII-digested lambda DNA 36 μL of HindIII-digested lambda DNA (NEB; 500 mg/mL) was de- phosphorylated with 2 μL of Calf Intestinal Phosphatase (NEB) in the appropriate buffer for 1 hour with shaking (1250 rpm) at 37°C. The samples were then purified with a High Pure PCR Product Purification Kit (Roche). Following this, the de-phosphorylated DNA (8 μL) was incubated with T4 Polynucleotide Kinase (2 μL) in the appropriate buffer, along with γ32P-dATP (3 μL) and H2O (5 μL) for 1 hour with shaking (1250 rpm) at 37°C. Unincorporated nucleotides were removed by passing the sample over two Illustra MicroSpin G-50 columns (GE Healthcare) and EDTA was added to a final concentration of 5 mM. The sample was stored at -20°C.
AutoNDA by SimpleDocs

Related to In Vitro DNA Replication Assays

  • SHOP DRAWINGS, PRODUCT DATA AND SAMPLES 4.12.1 Shop Drawings are drawings, diagrams, schedules and other, data specially prepared for the Work by the Contractor or any Subcontractor, manufacturer, supplier or distributor to illustrate some portion of the Work.

  • Treatment Program Testing The Employer may request or require an employee to undergo drug and alcohol testing if the employee has been referred by the employer for chemical dependency treatment or evaluation or is participating in a chemical dependency treatment program under an employee benefit plan, in which case the employee may be requested or required to undergo drug or alcohol testing without prior notice during the evaluation or treatment period and for a period of up to two years following completion of any prescribed chemical dependency treatment program.

  • Laboratory Testing All laboratories selected by UPS Freight for analyzing Controlled Substances Testing will be HHS certified.

  • MSAA Indicator Technical Specification Document This Agreement shall be interpreted with reference to the MSAA Indicator Technical Specifications document.

  • Program Components Activities and services delivered under this Program Element align with Foundational Programs and Foundational Capabilities, as defined in Oregon’s Public Health Modernization Manual, (xxxx://xxx.xxxxxx.xxx/oha/PH/ABOUT/TASKFORCE/Documents/public_health_modernization_man ual.pdf) as well as with public health accountability outcome and process metrics (if applicable) as follows:

  • Random Drug Testing All employees covered by this Agreement shall be subject to random drug testing in accordance with Appendix D.

  • Software Components At any time during the contract period of performance, the Government may require the Contractor to remedy any failure of the software to comply with the requirements of this contract. Support shall consist of correction of errors, provision of modifications, improvements, and other products the original manufacturer makes available to the Government without charge. The Government shall also be provided full documentation of changes and/or modifications to the software provided to meet the Government's requirements.

  • RE-WEIGHING PRODUCT Deliveries are subject to re- weighing at the point of destination by the Authorized User. If shrinkage occurs which exceeds that normally allowable in the trade, the Authorized User shall have the option to require delivery of the difference in quantity or to reduce the payment accordingly. Such option shall be exercised in writing by the Authorized User.

  • Diagnostic procedures to aid the Provider in determining required dental treatment.

  • Third Party Components The Products and Services may contain third party components (including open source software) subject to separate license agreements. To the limited extent a third party license expressly supersedes this XXXX, such third party license governs Customer’s use of that third party component.

Time is Money Join Law Insider Premium to draft better contracts faster.