COLLECTION AND HANDLING. Specimen: Serum or plasma from samples collected in EDTA may be used. Do not use plasma collected in heparin. Fresh serum is the specimen of choice.
COLLECTION AND HANDLING. Specimen: The specimen may be serum, cerebrospinal fluid (CSF) or urine. Serum specimens should be free of hemolysis or lipemia. A fibrinogen band, which may obscure the beta-gamma zone, will appear in plasma samples. Storage: Fresh serum is the specimen of choice. If storage is necessary, samples may be stored covered at 2 to 8°C for 48 hours.
COLLECTION AND HANDLING. Specimen: Freshly collected serum and CSF are the specimens of choice. Both serum and CSF should be collected at the same time using conventional collection methods for clinical laboratory testing. Handling: CSF samples should be applied neat. Serum samples should be diluted 1:300 in 0.85% saline. Storage and Stability: Samples can be stored refrigerated at 2 to 8°C for up to 3 days or 2 weeks at -20°C.
COLLECTION AND HANDLING. Use a 2.0 cc syringe to obtain the blood specimen as follows: Extracorporeal line: Using a two-syringe technique, flush the extracorporeal blood access line by withdrawing 2.0 cc of blood into a syringe and discarding it. Then use a second syringe to obtain a 0.5 cc sample for testing. In-dwelling line: Discontinue fluids drip, if necessary. Using a two-syringe technique, withdraw 2.0 cc of blood into a syringe and discard it. Then use a second syringe to obtain a 0.5 cc sample for testing. Venipuncture: Using a two-syringe technique, withdraw 2.0 cc of blood into a syringe and discard it. Then use a second syringe to obtain a 0.5 cc sample for testing. PROCEDURE
COLLECTION AND HANDLING. Specimen: Serum is the specimen of choice. Plasma collected in heparin may be used. Anticoagulants containing oxalate, citrate or EDTA cannot be used because these substances inhibit the alkaline phosphatase activity.13 Patient Preparation: The patient should be fasting. Patients who have B or O blood group and are secretors may have an elevated ALP about two hours after a fatty meal.6, 12, 13, 18, 19 Interfering Substances:
COLLECTION AND HANDLING. Specimen: Plasma from whole blood collected in sodium citrate as an anticoagulant. Specimen Preparation: Collect the blood specimen in either 3.2% (0.109 M) or 3.8% (0.129 M) sodium citrate. Add nine parts whole blood to one part sodium citrate solution. Centrifuge the blood sample immediately after collection at 1000 x G for 10 minutes. Store the plasma at 2 to 8°C until testing is performed. Plasma stored at 2 to 8°C must be tested within four hours after sample collection. Plasma is stable at -20°C for one month. Plastic tubes must be used for storage and testing. PROCEDURE
COLLECTION AND HANDLING. Specimen: Serum is the specimen of choice. Plasma from blood specimens collected in heparin or EDTA may be used. Anticoagulants containing oxalate should not be used due to the inhibition of LD by oxalate11. Plasma samples should be well centrifuged to eliminate platelets which contain LD12. Interfering Substances:
COLLECTION AND HANDLING. Specimen: Serum is the specimen of choice. Anticoagulants containing oxalate, citrate or EDTA cannot be used because these substances inhibit the alkaline phosphatase activity.17 Total alkaline phosphatase activity should be determined.
COLLECTION AND HANDLING. Specimen: Plasma obtained from whole blood collected with 3.2% sodium citrate as an anticoagulant is the specimen of choice. Specimen Collection: Blood may be collected with evacuated test tubes, a 2-syringe technique, or with a butterfly and syringe technique. Accurate coagulation studies depend on the correct whole blood to anticoagulant ratio. For blood specimens with hematocrits (HCT) of <55% (normal), 9 parts of freshly collected whole blood should be immediately added to one part anticoagulant.8 For blood specimens with hematocrits outside the normal range, adjust the amount of whole blood added to the anticoagulant according to the following formula.2 Parts whole blood to 0.6 one part anticoagulant (1 - .HCT) Particular care should be taken when using evacuated test tubes. These tubes are designed to draw 9 parts blood to 1 part anticoagulant. If the hematocrit is determined abnormal, blood should be drawn into a syringe and an appropriate amount mixed with an adjusted volume of citrate anticoagulant. Specimen Preparation: Centrifuge the whole blood specimen at 1600-2000 x G for 10 minutes. Immediately separate the plasma from the red blood cells, and place it in a plastic test tube with cap. Storage and Stability: Prior to testing, the plasma should be stored in the capped plastic tubes at 2 to 8°C. If testing is delayed for more than 2 hours, the plasma may be stored at -20°C or colder for up to one month. Thaw quickly at 37°C prior to testing, but do not allow to stand at 37°C for more than 5 minutes. PROCEDURE Materials Provided: Lyophilized Platelets - Cat. No. 5356 Materials Required but not provided in the kit: Platelet Aggregometer (AggRAM Analyzer recommended) Plastic pipette tips Plastic or siliconized test tubes Pipettes to deliver 50 µL or 25 µL Pipettes to deliver 400 µL or 200 µL STEP BY-STEP METHOD
COLLECTION AND HANDLING. Throughout the procedure for determination of activated partial thromboplastin times (aPTT) all test tubes, syringes and pipettes must be plastic or siliconized glass. Specimen: Plasma obtained from whole blood collected with sodium citrate as an anticoagulant is the specimen of choice. The concentration of the sodium citrate should be 3.8% (0.129 M) or 3.2% (0.109 M).
