TA cloning definition

TA cloning means the proprietary direct cloning system developed by LICENSOR for cloning the products of PCR processes including, but not limited to, the preparation of vectors with a single thymidine overhang on both ends to prevent self ligation and to accept DNA that has been synthesized by Taq polymerase leaving a single adenine overhang.
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Examples of TA cloning in a sentence

  • LICENSOR hereby grants to LICENSEE a field-of-use exclusive license to use the TA cloning technology including the know-how and trade secrets appurtenant thereto for the manufacture and sale of TA cloning products and kits anywhere in the world.

  • LICENSEE, at its discretion, may request LICENSOR to file an application for a patent in the United Sates or Canada on the TA cloning technology.

  • Should any additional products result from the research conducted by LICENSOR relating to TA cloning in the field of use described herein, LICENSOR shall grant to LICENSEE the right to negotiate in good faith the right to market those products.

  • LICENSOR agrees to inform LICENSEE in a confidential basis of any innovations or improvements to TA cloning that could improve the kit or would result in additional products.

  • LICENSOR reserves the right to and intends to submit for publication a scientific paper describing the TA cloning technology.

  • In consideration for the delivery of the know-how and trade secret information relating to TA cloning to LICENSEE, and the grant of right to manufacture and sell kits for TA cloning as described above, LICENSEE will pay an initial licensing fee to LICENSOR of * , due and payable in four (4) installments, * upon delivery of the trade secret and know-how information to LICENSEE, and an additional amount equal to * * due each successive thirty (30) days thereafter.

  • If such a paper is published, LICENSEE agrees, upon the written request of LICENSOR, to include a citation to the published paper in any printed advertisements or printed promotional pieces which LICENSEE may choose to print about its reagents or kits for the TA cloning technology.

  • In addition, LICENSEE shall pay to LICENSOR a royalty equal to * of net domestic sales of any reagents or kits incorporating the TA cloning technology as described herein and * * foreign sales of such regents or kits.

  • All fragments were cloned by using Topo TA cloning kit (Invitrogen).

  • Briefly, PCR amplicons corresponding to the regions showing dual probe reactivity in MHA were ligated into TOPO vector (TOPO TA cloning, Invitrogen, USA) carrying plasmid encoding antibiotic resistance, and then transformed into competent cells.