Examples of Gibco in a sentence
HeLa cells in complete media (Dulbecco’s modified Eagle medium (DMEM), Gibco, Carlsbad, California, USA; 5% fetal bovine serum (FBS), Gibco, Carlsbad, California, USA) were treated with 10 mg of exosomes, phosphatebuffered saline (PBS) (Gibco, Carlsbad, California, USA; neg- ative control), or pH9.0 alkaline buffer (positive control), and then incubated at 37○C with 5% carbon dioxide for 18 h.
Methods have been validated using Taq polymerase from Perkin Elmer (AmpliTaq) and Gibco BRL.
DMEM: Dulbecco’s Modified Eagle Medium powder, high glucose (Gibco BRL, Cat #12100-046)2.
Roswell Park Memorial Institute (RPMI)-1640 supplemented with L- glutamine and HEPES, fetal bovine serum (FBS), and penicillin– streptomycin (P/S, 10 000 IU mL—1) were purchased from Gibco BRL (Life Technologies Ltd., Paisley, UK).
CM was composed of αMEM with Glutamax and no nucleosides (Gibco) supplemented with 10% fetal bovine serum (FBS), without penicillin-streptomycin (PenStrep) at 37 °C and 5% CO2.
No. 12561056) was supplemented with fetal bovine serum (FBS, USA origin, Life Technologies, Australia), penicillin /streptomycin (Gibco), L-ascorbic acid-2-phosphate (Sigma-Aldrich), and 2mM L-Glutamine (Life Technologies, Australia).
Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM high glucose, Gibco), supplemented with 10% fetal calf serum (FCS), 100U penicillin and 100U streptomycin (LONZA) and maintained at 37°C with 5% CO2 atmosphere.
All chemicals used for tissue culture were purchased from Gibco BRL (Grand Island, USA).
The 24 well plate Transwell inserts were coated with 0.03 mg/mL Collagen I, Rat Tail (Gibco).
The skin tissue of the dead fetus was then collected and delivered to the laboratory in Dulbecco's Modified Eagle's medium (DMEM) medium (Gibco, 10569-010) supplemented with Penicillin/Streptomycin (P/S) (500 U/mL) (Gibco, 15140122) and 1% amphotericin B (Gibco, R01510) at 4℃.