Pest Management. 1. Spray low noxious pesticides to control the plant hoppers and borers 1-3 times during the vegetative phase. Appendix A — N6-P medium (mg per liter) N6 basal salt mixture (Sigma) 1 bag (1 liter size) KI 0.8 CoCI2×6H2O 0.025 XxXX0×0X0X 0.000 XxXxX0×0X0X 0.25 Glycine 2 Myo-inositol 000 Xxxxxxxx XXX 0 Xxxxxxxxxx XXX 1 Nicotinic acid 1 Proline 2,800 Casamino acid 300 Sucrose 30,000 2.4-D 2 pH 5.6 Gelrite 4,000 (For Bar marker and Biolaphos selection) Or Agarose 7,000-8,000 (For NPT II marker and Paramomycin selection) Autoclave at 121 °C for 15 min. Appendix B — N6-AS medium (mg per liter) N6 basal salt mixture (Sigma) 1 bag (1 liter size) KI 0.8 CoCI2×6H2O 0.025 XxXX0×0X0X 0.000 XxXxX0×0X0X 0.25 Glycine 2 Myo-inositol 000 Xxxxxxxx XXX 0 Xxxxxxxxxx XXX 1 Nicotinic acid 1 Casamino acid 1,000 Sucrose 30,000 Glucose 10,000 2.4-D 2 pH 5.6 Gelrite (semi-solid medium) 4,000 Autoclave at 121 °C for 15 min. Add in acetosyringone at the final concentration of 200 mM just before use.
Appears in 4 contracts
Samples: Model Agreement (Ceres, Inc.), Model Agreement (Ceres, Inc.), Model Agreement (Ceres, Inc.)
