DMSO definition

Examples of DMSO in a sentence

• Prepare an intermediate dilution series of each test compound by 4X serial dilution in DMSO such that the top concentration is 1 mM (suggested starting point) (Figure 6, Step 1).

• Prepare 3% DMSO control solution by adding 30 µL DMSO to 970 µL Kinase Buffer A.

• Add 60 µL of DMSO to 5 wells in each of 2 columns of a 96-well plate (wells A1 to E2).

• Add 5 µL of DMSO control solution to the other three wells for each tracer concentration (columns 4-6).

• Note L : The classification as a carcinogen need not apply if it can be shown that the substance contains less than 3 % DMSO extract as measured by IP 346 ‘Determination of polycyclic aromatics in unused lubricating base oils and asphaltene free petroleum fractions — Dimethyl sulphoxide extraction refractive index method’, Institute of Petroleum, London.

• Subtract the competitor curve (+ staurosporine) from the control curve (DMSO only) to correct for background signal, which is typically due to diffusion enhanced FRET from Eu to unbound tracer.

• Prepare 30 µM staurosporine (“competitor solution”) by diluting 30 µL of 1 mM staurosporine (from a stock in DMSO) into 970 µL Kinase Buffer A.

• Plot [tracer] versus emission ratio for the competitor (staurosporine) and control (DMSO only) (Figure 4).

• Prepare Fluo-8, AM (AAT Bioquest: 21080) Ca2+ dye by dissolving 1mg of Fluo-8 NW in 200 µL of DMSO.

• Substance / Mixture:Mixture Highly refined base oil (IP 346 DMSO extract < 3%).