Approach and Methodology. Provide a description of how the work will be performed and provide the proposed project schedule. Include any additional information or description of unique resources that are planned to be used to produce the deliverables. Include any proprietary technologies, methods or approaches that will be used on this project to improve quality or efficiency. If the proposal is for an IDIQ contract, the consultant should review the scope of services in Attachment A to the advertisement to obtain a general understanding of what a typical task order would entail. Based upon that understanding, the consultant should provide a sample schedule that identifies the major milestones, deliverables, tasks, etc., to demonstrate sufficient understanding of a typical task order. The duration of the task order is not required. This section shall be limited to four pages. If more than four pages are included, all pages after the fourth page will not be evaluated. CE&I Task 4: Construction Completion CE&I Task 5: Post-Constr. Acitivities All South’s plan begins with an Internal Review with DOTD. This first step is essential toward ensuring DOTD receives the outcome they desire. This Task is intended to establish confirm project goals and objectives and set project expectations. All South will request an advanced copy of the project plans and specifications from the DOTD project engineer and will take this opportunity to initially familiarize ourselves with the project particulars prior to meeting with DOTD. All South will conduct a plan-in-hand walk-through prior to meeting. All South will prepare a list of questions and observations based on this review and will use this list as draft topics for discussion during an initial meeting with DOTD. All South intends to finalize project goals and objectives during this meeting. Initial goals established during design may need to be refined prior to construction start. Project expectations will be established related to performance, reporting, communication, chain-of-command, and other general responsibility standards between All South and DOTD, and as defined in DOTD’s Standards and Procedures. Task 2 includes activities with the Contractor, All South, and DOTD to implement the project. These activities include developing an internal performance plan and conducting a pre-construction meeting. All South will develop an internal performance plan, identifying the roles of all internal team members. This plan will itemize project milestone...
Approach and Methodology. ARS will study experimental culture conditions for the ARS-PICM-19 cell line, its derivative cell lines, or other pig epiblast-derived liver cell lines (as described under ARS patent #5,532,156, “Hepatocyte Cell Line Derived from the Epiblast of Pig Blastocysts”) so as to optimize their hepatocyte functions for use as an in-vitro liver model, for their use in an artificial liver device, and for their use in the in-vitro assay of metabolic, toxic, or carcinogenic responses. Specific project objectives are the following:
1) Develop feeder-cell-independent and serum-free medium cell culture systems allowing the growth and differentiation of ARS-PICM-19 cells, or subclones or subpopulations of the ARS-PICM-19 cells, under defined conditions.
2) Develop spheroid cultures of PICM-19 cells without STO feeder cells and testing of rotating cell culture system (RCCS) for production and maintenance of spheroids.
3) Investigate effects of accessory cells obtained from pig liver on ARS-PICM-19 growth, differentiation, and metabolic function.
4) Assay ARS-PICM-19 cells and spheroids for liver specific functions by measuring P450 activity, γ-glutamyltranspeptidase activity, urea production, and ammonia clearance.
5) Assay ARS-PICM-19 liver specific protein synthesis and secretion by electrophoretic, immunochemical, or mass spectrophotometric techniques.
6) Develop and test, by in-vitro assay, flow-through bioreactors that enable the growth, differentiation, and maintenance of metabolic function of the ARS-PICM-19 cell line, or its derivative cell lines, over long term culture (1-3 months).
7) Develop and test multi-well cell culture formats for the in-vitro assay of the effects of various test compounds on the metabolism and viability of ARS-PICM-19-derived hepatocytes or bile ductules.
8) Genetically engineer ARS-PICM-19 cells to create derivative cell lines containing gene reporter constructs, e.g., green fluorescent protein (GFP) based constructs, so that GFP expression is linked to various cell metabolic responses or stimulation of various cell signal transduction pathways.
9) Develop cell transformation assay formats to demonstrate and enable the utilization of the ARS-PICM-19 cell line for the study of mutagenic or carcinogenic processes. Hepalife will provide funds for the salary of one post-doctoral researcher, one support scientist, and one technician for a period of three years and funds for the associated laboratory supplies and professional activities involve...
Approach and Methodology. The Tender Committee will in particular consider the following: Is the proposed approach and methodology robust and adequate to meet the objectives of the assignment? Is the description clear and concise and is the description tailored specifically to the assignments? Does the Tenderer show understanding for the need to engage TRA in the solutions and do the Tenderer propose adequate methods to ensure the engagement of TRA? Does the approach and methodology reflect command of the subject matter, both in terms of knowledge and understanding of the current context? Further more, when relevant, the evaluation will consider whether potential challenges or special issues have been identified, and whether the solutions for overcoming these are satisfactory The Tender Committee will in particular consider the following: Does the description show that the Tenderer proposes technically adequate methods when identifying the challenges stipulated in the cases? Does the description show, that the Tenderer is able to identify the challenges and propose adequate and realistic solutions to the challenges? Does the description show that the Tenderer understands and carefully consider the maturity of TRA? Does the description show, that the Tenderer is able to balance the effort required by TRA and the need for progress in the projects? The Tender Committee will in particular consider the following: The proposed organization (for the solution of the technical cases) is efficient with clear lines of responsibility and communication both internally (at the Tenderer itself) and in relation to the recipient The completed Schedule 2.2 will be considered when evaluating the proposed Organization.
Approach and Methodology. Provide a detailed description of your approach and methodology to provide the TMS as described in Section 2.0 – Scope of Services. Ensure your responses include a project plan with timelines, a detailed explanation for accomplishing the specific work and deliverables. The response shall include information that will provide DHS with a clear understanding that your agency has the ability to meet or exceed the services requested in this WOS, at a minimum, describe how your agency will provide the following:
8.3.1 Develop a training plan inclusive of schedules, registration and attendance rosters for the variety of online, external (various location throughout the County) and/or internal (at the Respondent’s location) trainings in collaboration with the County.
8.3.2 Coordinate training needs with the County and other agencies identified by the County.
8.3.3 Develop, prepare, and obtain training curriculum, educational aids, materials (e.g., job aides, resource guides, program specific “program guides”, etc.), and any other supporting materials or literature that assist trainees in the full understanding of the training, as requested by the County.
8.3.4 Procure all requested trainings and update on an ongoing basis the trainings in Exhibit 1 – Training Classification Catalog.
8.3.5 Secure all web-based (e.g., Zoom, GoToMeeting, etc.) and in- person training platforms and/or venues, including recording of trainings session as requested by the County.
8.3.6 Secure and schedule internal and external trainers who are subject matter experts and are well versed on the applicable trainings identified in Exhibit 1 – Training Classification Catalog.
8.3.7 Provide support staff, for all trainings.
8.3.8 Organize training sessions for groups as small as 5 and up to a maximum of 500 trainees.
8.3.9 Manage and identify suitable venues with ample parking and provide classroom and/or conference room options for onsite trainings.
8.3.10 Coordinate the delivery of and/or print production of County approved training materials.
8.3.11 Identify and provide equipment needed for each training (e.g., projection system and white board with markers) for onsite trainings.
8.3.12 Provide internet access, computers, and other technical equipment necessary for onsite trainings.
8.3.13 Provide onsite technical and computer support at the training venue(s).
8.3.14 Provide web-based technical support for online trainings.
8.3.15 Provide sign-in sheets for each training session and/or main...
Approach and Methodology. ARS will study culture conditions for the ARS-PICM-19 cell line, its derivative cell lines, or other pig epiblast-derived liver cell lines (as described under ARS patent #5,532,156, “Hepatocyte Cell Line Derived from the Epiblast of Pig Blastocysts”) so as to optimize their hepatocyte functions for use as an in vitro liver model, for their use in a flow-through bioreactor (“HepaLife bioreactor”), a multicompartment flow-through hollow-fiber bioreactor currently manufactured by Stem Cell Systems GmbH, Germany, or any other bioreactor design designated by HepaLife Biosystems, Inc., and for their use in the in vitro assay of metabolic or toxic responses.
Approach and Methodology. (Be sure to cover the activities of both ARS and COOPERATOR.)
Approach and Methodology. The consultant is expected to develop a review plan based on the suggested review questions above but may suggest additional questions or modifications. The consultant will answer the review questions using a mixed approach including: - Review of project reports. - Discussion with technical and financial experts associated with the project (Workshop approach and one-on-one discussions where appropriate). - Visit to project sites. - Individual and group interviews with stakeholders. - Other approaches and methodologies as appropriate. The consultant should ensure that the proposed methodology adopts consultative and participative approaches to ensure that all the key stakeholders are involved.
Approach and Methodology. The following is a summary of the approach and methodology to be applied:
Approach and Methodology. Provide a clear and concise description of the approach and methodology OWeror proposes to complete all code review services as described in the Scope of Work.
Approach and Methodology. 8.3.1 General Approach: A description of the overall approach and strategy to this project.
