STEP METHOD Sample Clauses

STEP METHOD. NOTE: If the staining chamber was last used to stain a gel, the SPIFE Touch software has an automatic wash cycle prompted by the initiation of the SPIFE Touch LD Isoenzyme test. To verify the status of the stainer chamber, use the arrows under the STAINER UNIT to select the appropriate test, place the empty Gel Holder into the stainer chamber and press START. If washing of the staining chamber is necessary, the prompt “Vat must be washed. Remove gel and install gel holder.” will appear. Press RETRY to begin the stainer wash. The cleaning process will complete automatically in about 7 minutes. To avoid delays after incubation, this wash cycle should be initiated at least 7 minutes prior to the end of the run.
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STEP METHOD. I. Instrument Parameters Using the instructions provided in the Operator’s Manual, set up parameters as follows: Separator Unit
STEP METHOD. I. Chamber Preparation
STEP METHOD. I. Calibration
STEP METHOD. NOTE: If a SPIFE procedure requiring a stain has been run prior to running the LD gels, the stainer unit must be cleaned/washed before washing the gel. The new software version 1.20 has an automatic wash cycle prompted by initiation of a test which does not use the stainer unit for staining when the previous test did use the stainer for staining. To avoid delays after elec- trophoresis, this wash cycle should be initiated at least seven (7) minutes prior to the end of the run. To verify the status, press the TEST SELECT/ CONTINUE button on the stainer until the appropriate test is selected. Place an empty Gel Holder in the stainer unit. If cleaning is required, the “Wash 1” prompt will appear, followed by “Plate out, Holder in” prompts. Press “Continue” to begin the stainer wash. The cleaning process will complete automatically in about 7 minutes. The unit is then ready to process the gel after incubation. Reagent Preparation Reconstitute two vials of the LD Isoenzyme Reagent with 1.0 mL LD Isoenzyme Diluent each. Mix well by inversion. Sample Preparation If testing 41-60 samples, remove three Applicator Blades from the packag- ing. If testing fewer samples, remove the appropriate number of Applicator Blades from the packaging. Place the three Applicator Blades into the vertical slots in the Applicator Assembly identified as 2, 9 and 16. If using fewer Applicator Blades, place them into any of the three slots noted above. Load Sample 1 00:02 20°C SPD6 No Prompt Load Sample 2 00:02 20°C SPD6 No Prompt Load Sample 3 00:02 20°C SPD6 No Prompt Load Sample 4 00:30 20°C SPD6 No Prompt Apply Sample 1 00:30 20°C SPD6 LOC1 No Prompt Electrophoresis 1 6:00 10°C 600 Volt 100mA No Prompt Apply Reagent 1 45°C 4 cycles To Continue, (Continue) Incubate 1 20:00 45°C No Prompt END OF TEST Stainer Unit No Prompt Destain 1 15:00 REC=REV VALVE=2 No Prompt Wash 1 10:00 REC=REV VALVE=7 No Prompt Dry 1 25:00 70°C No Prompt END OF TEST Electrophoresis Open the chamber lid. Place the Cup Tray with samples on the SPIFE 3000. Align the holes in the tray with the pins on the instrument. Place a reconstituted vial of reagent into each outer hole of the reagent bar, ensuring that the vials are pushed down as far as they can go. Close the chamber lid. With LD on the display, press the START/STOP button. An option to either begin the test or skip the operation will be presented. Press START/STOP to begin. The SPIFE 3000 will apply the samples, electrophorese, spread reagent and beep. Ope...

Related to STEP METHOD

  • Measurement method An isolation resistance test instrument is connected between the live parts and the electrical chassis. The isolation resistance is subsequently measured by applying a DC voltage at least half of the working voltage of the high voltage bus. If the system has several voltage ranges (e.g. because of boost converter) in conductively connected circuit and some of the components cannot withstand the working voltage of the entire circuit, the isolation resistance between those components and the electrical chassis can be measured separately by applying at least half of their own working voltage with those components disconnected.

  • Billing Method 1.5.1 To receive payment for services rendered under this contract, the Contractor shall submit a fully completed invoice for work previously performed to: Minneapolis Public Housing Authority Attention: Accounts Payable, Suite 307 0000 Xxxxxxxxxx Xxx X, Xxxxxxxxxxx, XX 00000 or: xxxxxxxx@xxxxxxx.xxx

  • ICE Method The method used to calculate One-Month LIBOR, as described in Section 3.05.

  • Payment Methodology The Contractor shall be compensated based on the Service Rates in Attachment for units of service authorized by the Institution in a total amount not to exceed the Contract Maximum Liability established in Section C.1. The Contractor’s compensation shall be contingent upon the satisfactory completion of units of service or project milestones identified in Attachment B. The Contractor shall submit invoices, in form and substance acceptable to the Institution with all of the necessary supporting documentation, prior to any payment. Such invoices shall be submitted for completed units of service or project milestones for the amount stipulated.

  • Calculation methodology No adjustment in the Conversion Price need be made unless the adjustment would require an increase or decrease of at least 1% in the Conversion Price then in effect, provided that any adjustment that would otherwise be required to be made shall be carried forward and taken into account in any subsequent adjustment. Except as stated in this Article VI, the Conversion Rate will not be adjusted for the issuance of Common Stock or any securities convertible into or exchangeable for Common Stock or carrying the right to purchase any of the foregoing. Any adjustments that are made shall be carried forward and taken into account in any subsequent adjustment. All calculations under Article V and Section 6.06 hereof and this Section 6.07 shall be made to the nearest cent or to the nearest 1/10,000th of a share, as the case may be.

  • Balance Computation Method For all dividend-bearing Accounts, dividends are calculated by the average daily balance method which applies a daily periodic rate to the average daily balance for the average daily balance calculation period. The average daily balance is determined by adding the full amount of the principal in Your Account for each day of the period and dividing that figure by the number of days in the period. Accrual on Noncash Deposits. For dividend-bearing Accounts, dividends will begin to accrue on the business day that You deposit noncash items (e.g. checks) into Your Account.

  • Voting Method Unless otherwise provided by law, when a vote is taken upon any measure before the Board, with a quorum being present, a majority of the votes cast shall determine its outcome. A vote of "abstain" or "present," or a vote other than "yea" or "nay," or a failure to vote, is counted for the purposes of determining whether a quorum is present. A vote of "abstain" or "present," or a vote other than "yea" or "nay," or a failure to vote, however, is not counted in determining whether a measure has been passed by the Board, unless otherwise stated in law. The sequence for casting votes is rotated. On all questions involving the expenditure of money and on all questions involving the closing of a meeting to the public, a roll call vote shall be taken and entered in the Board's minutes. An individual Board member may request that a roll call vote be taken on any other matter; the President or other presiding officer may approve or deny the request but a denial is subject to being overturned by a majority vote of the members present. Any Board member may request that his or her vote be changed before the President announces the result. Any Board member may include a written explanation of his or her vote in the District file containing individual Board member statements; the explanation will not be part of the minutes. Minutes The Board Secretary shall keep written minutes of all Board meetings (whether open or closed), which shall be signed by the President and the Secretary. The minutes include:

  • Payment Method Payment shall be made by the Contractor to the Subcontractor as follows: (choose one) ☐ - Immediately upon completion of the Services to the satisfaction of the Contractor. ☐ - Within business days after completion of the Services to the satisfaction of the Contractor. ☐ - Shall be paid on a ☐ weekly ☐ monthly ☐ quarterly ☐ other

  • PayPal as Login Method We may allow you to authenticate with PayPal when you log into certain external websites or mobile apps. If we do so, we may share your login status with any third party enabling you to log in in this way, as well as the personal and other account information that you consent to being shared so that the third party can recognize you. PayPal will not give the third party access to your account and will only make payments from your account to that third party with your specific authorization and instruction. If you enable visitors to authenticate with PayPal when they log into your website, app, or your customer accounts, you must agree to any specific terms applicable when this functionality is made available to you, and comply with any specifications in any integration manual or guideline. We do not guarantee or otherwise represent the identity of any user of this login method. We will not share with you the personal and other account information of the user (including login status) held by PayPal unless the user has consented to our disclosure of that information to you. Corporate Customers When we refer to “PSD2” in this section we mean the Second EU Payment Services Directive ((EU)2015/2366). We consider you to be a “Corporate Customer” if, on the date you entered into this user agreement, you are not: • A consumer, (being an individual acting for purposes other than a trade, business or profession). • A micro-enterprise (being an enterprise which employs fewer than 10 persons and has an annual balance sheet that does not exceed 2 million EUR. We and you may agree that certain provisions of PSD2 (as implemented by local law) do not apply to your use of our service if you are a Corporate Customer. If you are a Corporate Customer you agree that: • You are not entitled to a refund for billing agreement payments. • Where you identify a problem you have up to 60 (sixty) days from the date on which the problem happened to notify us about it, after which time we have no obligation to investigate and refund you. • We are not obliged to comply with the information requirements set out in Title III of PSD2 and their equivalents in any implementation of PSD2 in member states of the European Economic Area that may apply to you (“PSD2 transpositions”). • Articles 72 and 89 of PSD2 and equivalent provisions in PSD2 transpositions do not apply to your use of our service, meaning that, even where we may say so otherwise in this user agreement, we are not liable to you for the losses or damage you may suffer under those articles and provisions. Section numbers and defined words

  • Deployment Method You may only install the Products on servers and workstations under the control of your third-party service provider who hosts the Products on your behalf in a public cloud, and will deploy the Products only in the operating environment specified on the order. If the “Cloud Platform for AWS” operating environment is specified on the order, you may deploy the Products listed on the order solely in an Amazon Web Services environment, except for any Products which technically require deployment in a different operating environment. If the “Cloud Platform for Azure” operating environment is specified on the order, you may deploy the Products listed on the order solely in a Microsoft Azure environment, except for any Products which technically require deployment in a different operating environment. 4. 部署方式。您只能在代表您于公共云中托管产品的第三方服务提供商控制的服务器或工作站上安装产品,并且您将只在订单指定的操作环境中部署产品。如果订单上指定了“Cloud Platform for AWS”操作环境,您仅可以在Amazon Web Services 环境中部署订单上所列之产品,因技术要求必须被部署在其他操作环境中的产品除外。 如果订单上指定了“Cloud Platform for Azure”操作环境,则您仅可以在 Microsoft Azure 环境中部署订单上所列之产品,因技术要求必须被部署在其他操作环境中的产品除外。

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